Molecular analysis of streptomycin-resistance associating genes in Mycobacterium tuberculosis isolates from Nepal

Shrestha, Dipti; Maharjan, Bhagwan; Oo, Nan Aye Thida; Isoda, Norikazu; Nakajima, Chie; Suzuki, Yasuhiko

Molecular analysis of streptomycin-resistance associating genes in Mycobacterium tuberculosis isolates from Nepal

Shrestha, Dipti; Maharjan, Bhagwan; Oo, Nan Aye Thida et al.

Tuberculosis, 2020, 125, 101985

Permalink : https://hdl.handle.net/2115/83725

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論文情報

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アクセス権	open access
URI	https://hdl.handle.net/2115/83725
タイトル	Molecular analysis of streptomycin-resistance associating genes in Mycobacterium tuberculosis isolates from Nepal
著者	Shrestha, Dipti ; Maharjan, Bhagwan ; Oo, Nan Aye Thida ; Isoda, Norikazu ; Nakajima, Chie ; Suzuki, Yasuhiko
著者名 Shrestha, Dipti
著者名 Maharjan, Bhagwan
著者名 Oo, Nan Aye Thida
e-Rad_Researcher 80615732 著者名 Isoda, Norikazu
e-Rad_Researcher 60435964 著者名 Nakajima, Chie
e-Rad_Researcher 90206540 著者名 Suzuki, Yasuhiko
言語	英語
キーワード	Mycobacterium tuberculosis
	Streptomycin
	Drug-resistance
	rpsL
	rrs
	gidB
発行日	2020-12
出版者	Elsevier
収録物名	Tuberculosis
巻(号)	125
ページ	101985
抄録	Mutation in rpsL (encoding ribosomal protein S12), rrs (encoding 16S ribosomal RNA) and gidB (encoding 7-methylguanosine methyltransferase) are associated with resistance to streptomycin (STR), which is used for the treatment of multi-drug resistant tuberculosis (MDR-TB) in Nepal. The aim of our study is to analyze the correlation between mutations in the target genes and STR-resistance in 197 Mycobacterium tuberculosis (MTB) isolates from Nepal. Mutations in rpsL was harbored by 65.9% of isolates, in which the most common mutation in rpsL is caused by K43R (58.8%) and were significantly associated with Beijing genotype (P < 0.001). About 13.2% of isolates harbored mutations in two highly mutable regions of rrs, the 530 loop and the 912 region. About 13.2% of gidB mutants do not show any mutation in rpsL and rrs, which might suggest the role of gidB mutations in STR-resistance in MTB. In addition, 5.6% of isolates do not show any mutations in three genes examined, suggesting the involvement of other mechanism in STR-resistance in MTB. Our findings can be implemented for the establishment of molecular STR-susceptibility testing, in which tuberculosis can be treated with appropriate drugs and can improve control strategies for DR-TB.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
権利情報	© 2020. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
PISSN	1472-9792
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.tube.2020.101985