Gut commensals suppress interleukin-2 production through microRNA-200/BCL11B and microRNA-200/ETS-1 axes in lamina propria leukocytes of murine large intestine

Ohsaka, Fumina; Karatsu, Yugo; Kadota, Yoshihiro; Tochio, Takumi; Takemura, Naoki; Sonoyama, Kei

Gut commensals suppress interleukin-2 production through microRNA-200/BCL11B and microRNA-200/ETS-1 axes in lamina propria leukocytes of murine large intestine

Ohsaka, Fumina; Karatsu, Yugo; Kadota, Yoshihiro et al.

Biochemical and biophysical research communications, 2021, 534, 808-814

Permalink : https://hdl.handle.net/2115/83740

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論文情報

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アクセス権	open access
URI	https://hdl.handle.net/2115/83740
タイトル	Gut commensals suppress interleukin-2 production through microRNA-200/BCL11B and microRNA-200/ETS-1 axes in lamina propria leukocytes of murine large intestine
著者	Ohsaka, Fumina ; Karatsu, Yugo ; Kadota, Yoshihiro ; Tochio, Takumi ; Takemura, Naoki ; Sonoyama, Kei
著者名 Ohsaka, Fumina
著者名 Karatsu, Yugo
著者名 Kadota, Yoshihiro
著者名 Tochio, Takumi
著者名 Takemura, Naoki
e-Rad_Researcher 90241364 著者名 Sonoyama, Kei
言語	英語
キーワード	Gut microbiota
	MicroRNA
	BCL11B
	ETS-1
	Interleukin-2
	Large intestine
発行日	2021-01-01
出版者	Elsevier
収録物名	Biochemical and biophysical research communications
巻(号)	534
ページ	808-814
抄録	The role of microRNAs (miRNAs) in how microbiota influence the host intestinal immune system is not fully understood. We compared the expression profiles of miRNAs and mRNAs in lamina propria leukocytes (LPL) in the large intestines of germ-free (GF) and specific pathogen-free (SPF) mice. Microarray analysis revealed different expression profiles of miRNAs and mRNAs between GF and SPF mice. Quantitative real time-PCR (qRT-PCR) showed that the level of miR-200 family members was significantly higher in SPF mice than in GF mice. In silica prediction followed by qRT-PCR suggested that Bcl11b, Ets1, Gbp7, Stat5b, and Zeb1 genes were downregulated by the miR-200 family. Western blotting revealed that the expression of BCL11B and ETS-1, but not ZEB1, in large intestinal LPL was significantly lower in SPF mice than in GF mice. Interleukin (IL)-2 production in cultured LPL upon stimulation with phorbol 12-myristate 13-acetate and ionomycin for 24 h was significantly lower in SPF mice than in GF mice. Conventionalization of GF mice substantially recapitulated SPF mice in terms of the expression of miR200 family members and their target genes and IL-2 production in large intestinal LPL. Considering that BCL11B and ETS-1 reportedly function as transcription factors to activate the Il2 gene, we propose that the presence of gut commensals suppresses IL-2 production in large intestinal LPL, at least in part through post-transcriptional downregulation of Bcl11b and Ets1 genes by miR-200 family members. (C) 2020 Elsevier Inc. All rights reserved.
資源タイプ	学術雑誌論文
出版タイプ	Author's Original
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
権利情報	©2021. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
PISSN	0006-291X
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.bbrc.2020.10.103