Bioimaging of Pb and STIM1 in mice liver, kidney and brain using Laser Ablation Inductively Coupled Plasma Mass Spectrometry (LA-ICP-MS) and immunohistochemistry

Togao, Masao; Nakayama, Shouta M. M.; Ikenaka, Yoshinori; Mizukawa, Hazuki; Makino, Yoshiki; Kubota, Ayano; Matsukawa, Takehisa; Yokoyama, Kazuhito; Hirata, Takafumi; Ishizuka, Mayumi

Bioimaging of Pb and STIM1 in mice liver, kidney and brain using Laser Ablation Inductively Coupled Plasma Mass Spectrometry (LA-ICP-MS) and immunohistochemistry

Togao, Masao; Nakayama, Shouta M. M.; Ikenaka, Yoshinori et al.

Chemosphere, 2020, 238, 124581

Permalink : https://hdl.handle.net/2115/83751

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アクセス権	open access
URI	https://hdl.handle.net/2115/83751
タイトル	Bioimaging of Pb and STIM1 in mice liver, kidney and brain using Laser Ablation Inductively Coupled Plasma Mass Spectrometry (LA-ICP-MS) and immunohistochemistry
著者	Togao, Masao ; Nakayama, Shouta M. M. ; Ikenaka, Yoshinori ; Mizukawa, Hazuki ; Makino, Yoshiki ; Kubota, Ayano ; Matsukawa, Takehisa ; Yokoyama, Kazuhito ; Hirata, Takafumi ; Ishizuka, Mayumi
著者名 Togao, Masao
著者名 Nakayama, Shouta M. M.
著者名 Ikenaka, Yoshinori
著者名 Mizukawa, Hazuki
著者名 Makino, Yoshiki
著者名 Kubota, Ayano
著者名 Matsukawa, Takehisa
著者名 Yokoyama, Kazuhito
著者名 Hirata, Takafumi
e-Rad_Researcher 50332474 著者名 Ishizuka, Mayumi
言語	英語
キーワード	Cell entry mechanisms
	Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS)
	Distribution
	Pb
	Stromal interacting protein 1 (STIM1)
発行日	2020-01-01
出版者	Elsevier
収録物名	Chemosphere
巻(号)	238
ページ	124581
抄録	Lead (Pb) pollution is one of the most serious environmental problems and has attracted worldwide attention. Pb causes hematological, central nervous system, as well as renal toxicity, and so on. Although many investigations about Pb in blood to evaluate pollution status and toxic effects have been reported, there are open question about biological behavior of Pb. In order to reveal any toxicological mechanisms or influences, we focused on the local distribution of Pb in mice organs. Lead acetate (100 mg/L and 1000 mg/L) in drinking water were given to the BALB/c mice (male, seven weeks of age, N = 24) for three weeks. Laser Ablation Inductively Coupled Plasma Mass Spectrometry (LA-ICP-MS) analysis revealed a homogenous distribution of Pb in the liver and inhomogeneous distribution in the kidney and brain. The hippocampus, thalamus, and hypothalamus had higher concentrations than other areas such as the white matter. Surprisingly, in the kidney, Pb tended to accumulate in the medulla rather than the cortex, strongly suggesting that high sensitivity areas and high accumulation areas differ. Moreover, distribution of stromal interacting protein 1 (STIM1) which is candidate gene of Pb pathway to the cells was homogenous in the liver and kidney whereas inhomogeneous in the brain. In contrast to our hypothesis, interestingly, Pb exposure under the current condition did not induce mRNA expressions for any candidate channel or transporter genes. Thus, further study should be conducted to elucidate the local distribution of Pb and other toxic metals, and pathway that Pb takes to the cells. (C) 2019 Elsevier Ltd. All rights reserved.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
権利情報	© 2020. This manuscript version is made available under the CC-BY-NC-ND 4.0 license　https://creativecommons.org/licenses/by-nc-nd/4.0/
PISSN	0045-6535
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.chemosphere.2019.124581