Propolis suppresses cytokine production in activated basophils and basophil-mediated skin and intestinal allergic inflammation in mice

Kashiwakura, Jun-ichi; Yoshihara, Mari; Saitoh, Kodai; Kagohashi, Kota; Sasaki, Yuto; Kobayashi, Fuki; Inagaki, Iori; Kitai, Yuichi; Muromoto, Ryuta; Matsuda, Tadashi

Propolis suppresses cytokine production in activated basophils and basophil-mediated skin and intestinal allergic inflammation in mice

Kashiwakura, Jun-ichi; Yoshihara, Mari; Saitoh, Kodai et al.

Allergology international, 2020, 70(3), 360-367

Permalink : https://hdl.handle.net/2115/83838

このアイテムのアクセス数:387件（2026-01-18 10:29 集計）

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https://doi.org/10.1016/j.alit.2020.11.005

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アクセス権	metadata only access
URI	https://hdl.handle.net/2115/83838
タイトル	Propolis suppresses cytokine production in activated basophils and basophil-mediated skin and intestinal allergic inflammation in mice
著者	Kashiwakura, Jun-ichi ; Yoshihara, Mari ; Saitoh, Kodai ; Kagohashi, Kota ; Sasaki, Yuto ; Kobayashi, Fuki ; Inagaki, Iori ; Kitai, Yuichi ; Muromoto, Ryuta ; Matsuda, Tadashi
e-Rad_Researcher 90373290 著者名 Kashiwakura, Jun-ichi
著者名 Yoshihara, Mari
著者名 Saitoh, Kodai
著者名 Kagohashi, Kota
著者名 Sasaki, Yuto
著者名 Kobayashi, Fuki
著者名 Inagaki, Iori
e-Rad_Researcher 90756165 著者名 Kitai, Yuichi
e-Rad_Researcher 30455597 著者名 Muromoto, Ryuta
e-Rad_Researcher 20212219 著者名 Matsuda, Tadashi
言語	英語
キーワード	Basophils
	Food allergy
	IgE
	IL-4
	Propolis
発行日	2020-12-02
出版者	Japanese Society of Allergology
収録物名	Allergology international
巻(号)	70(3)
ページ	360-367
抄録	Background: Propolis is a resinous mixture produced by honey bees that contains cinnamic acid derivatives and flavonoids. Although propolis has been reported to inhibit mast cell functions and mast cell-dependent allergic responses, the effect of propolis on basophil biology remains unknown. This study aimed to investigate the inhibitory effect of propolis on Fc epsilon RI-mediated basophil activation. Methods: To determine the inhibitory effect of propolis on basophil activation in vitro, cytokine production and Fc epsilon RI signal transduction were analyzed by ELISA and western blotting, respectively. To investigate the inhibitory effect of propolis in vivo, IgE-CAI and a food allergy mouse model were employed. Results: Propolis treatment resulted in the suppression of IgE/antigen-induced production of IL-4, IL-6 and IL-13 in basophils. Phosphorylation of Fc epsilon RI signaling molecules Lyn, Akt and ERK was inhibited in basophils treated with propolis. While propolis did not affect the basophil population in the treated mice, propolis did inhibit IgE-CAI. Finally, ovalbumin-induced intestinal anaphylaxis, which involves basophils and basophil-derived IL-4, was attenuated in mice prophylactically treated with propolis. Conclusions: Taken together, these results demonstrate the ability of propolis to suppress IgE-dependent basophil activation and basophil-dependent allergic inflammation. Therefore, prophylactic treatment with propolis may be useful for protection against food allergic reactions in sensitive individuals.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
PISSN	1323-8930
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1016/j.alit.2020.11.005