Silencing of p53 and CDKN1A establishes sustainable immortalized megakaryocyte progenitor cells from human iPSCs

Sone, Masamitsu; Nakamura, Sou; Umeda, Sachiko; Ginya, Harumi; Oshima, Motohiko; Kanashiro, Maria Alejandra; Paul, Sudip Kumar; Hashimoto, Kanae; Nakamura, Emiri; Harada, Yasuo; Tsujimura, Kyoko; Saraya, Atsunori; Yamaguchi, Tomoyuki; Sugimoto, Naoshi; Sawaguchi, Akira; Iwama, Atsushi; Eto, Koji; Takayama, Naoya

Silencing of p53 and CDKN1A establishes sustainable immortalized megakaryocyte progenitor cells from human iPSCs

Sone, Masamitsu; Nakamura, Sou; Umeda, Sachiko et al.

Stem Cell Reports, 2021, 16(12), 2861-2870

Permalink : https://hdl.handle.net/2115/83898

このアイテムのアクセス数:480件（2026-01-15 10:15 集計）

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https://doi.org/10.1016/j.stemcr.2021.11.001

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アクセス権	metadata only access
URI	https://hdl.handle.net/2115/83898
タイトル	Silencing of p53 and CDKN1A establishes sustainable immortalized megakaryocyte progenitor cells from human iPSCs
著者	Sone, Masamitsu ; Nakamura, Sou ; Umeda, Sachiko ; Ginya, Harumi ; Oshima, Motohiko ; Kanashiro, Maria Alejandra ; Paul, Sudip Kumar ; Hashimoto, Kanae ; Nakamura, Emiri ; Harada, Yasuo ; Tsujimura, Kyoko ; Saraya, Atsunori ; Yamaguchi, Tomoyuki ; Sugimoto, Naoshi ; Sawaguchi, Akira ; Iwama, Atsushi ; Eto, Koji ; Takayama, Naoya
e-Rad_Researcher 90599771 著者名 Sone, Masamitsu
著者名 Nakamura, Sou
著者名 Umeda, Sachiko
著者名 Ginya, Harumi
著者名 Oshima, Motohiko
著者名 Kanashiro, Maria Alejandra
著者名 Paul, Sudip Kumar
著者名 Hashimoto, Kanae
著者名 Nakamura, Emiri
著者名 Harada, Yasuo
著者名 Tsujimura, Kyoko
著者名 Saraya, Atsunori
著者名 Yamaguchi, Tomoyuki
著者名 Sugimoto, Naoshi
著者名 Sawaguchi, Akira
著者名 Iwama, Atsushi
著者名 Eto, Koji
著者名 Takayama, Naoya
言語	英語
発行日	2021-12-14
出版者	Cell Press
収録物名	Stem Cell Reports
巻(号)	16(12)
ページ	2861-2870
抄録	Platelet transfusions are critical for severe thrombocytopenia but depend on blood donors. The shortage of donors and the potential of universal HLA-null platelet products have stimulated research on the ex vivo differentiation of human pluripotent stem cells (hPSCs) to platelets. We recently established expandable immortalized megakaryocyte cell lines (imMKCLs) from hPSCs by transducing MYC, BMI1, and BCL-XL (MBX). imMKCLs can act as cryopreservable master cells to supply platelet concentrates. However, the proliferation rates of the imMKCLs vary with the starting hPSC clone. In this study, we reveal from the gene expression profiles of several MKCL clones that the proliferation arrest is correlated with the expression levels of specific cyclin-dependent kinase inhibitors. Silencing CDKN1A and p53 with the overexpression of MBX was effective at stably inducing imMKCLs that generate functional platelets irrespective of the hPSC clone. Collectively, this improvement in generating imMKCLs should contribute to platelet industrialization and platelet biology.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
PISSN	2213-6711
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1016/j.stemcr.2021.11.001