Point-of-care molecular diagnosis of Mycoplasma pneumoniae including macrolide sensitivity using quenching probe polymerase chain reaction

Ishiguro, Nobuhisa; Sato, Rikako; Mori, Toshihiko; Tanaka, Hiroshi; Narita, Mitsuo; Nagano, Takashi; Owaku, Masato; Miyajima, Kensuke; Manabe, Atsushi

Point-of-care molecular diagnosis of Mycoplasma pneumoniae including macrolide sensitivity using quenching probe polymerase chain reaction

Ishiguro, Nobuhisa; Sato, Rikako; Mori, Toshihiko et al.

PLoS ONE, 2022, 16(10), e0258694

Permalink : https://hdl.handle.net/2115/83947

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アクセス権	open access
URI	https://hdl.handle.net/2115/83947
タイトル	Point-of-care molecular diagnosis of Mycoplasma pneumoniae including macrolide sensitivity using quenching probe polymerase chain reaction
著者	Ishiguro, Nobuhisa ; Sato, Rikako ; Mori, Toshihiko ; Tanaka, Hiroshi ; Narita, Mitsuo ; Nagano, Takashi ; Owaku, Masato ; Miyajima, Kensuke ; Manabe, Atsushi
e-Rad_Researcher 40168216 著者名 Ishiguro, Nobuhisa
著者名 Sato, Rikako
著者名 Mori, Toshihiko
著者名 Tanaka, Hiroshi
著者名 Narita, Mitsuo
著者名 Nagano, Takashi
著者名 Owaku, Masato
著者名 Miyajima, Kensuke
e-Rad_Researcher 20292849 著者名 Manabe, Atsushi
言語	英語
発行日	2022-01-28
出版者	PLOS
収録物名	PLoS ONE
巻(号)	16(10)
ページ	e0258694
抄録	Objectives Macrolides are generally considered to be the drugs of choice for treatment of patients with Mycoplasma pneumoniae infection. However, macrolide-resistant M. pneumoniae has been emerging since about 2000. The Smart Gene system (MIZUHO MEDY Co., Ltd., Tosu, Japan) is a novel fully automated system for detection of pathogens using the method of quantitative polymerase chain reaction (qPCR) with QProbe (QProbe PCR). The entire procedure is completed within 50 min and the size of the instrument is small (15 x 34 x 30 cm). The purpose of this study was to evaluate the usefulness of the Smart Gene system for detection of M. pneumoniae and detection of a point mutation at domain V of the 23S rRNA gene of M. pneumoniae. Materials Pharyngeal swab samples were collected from 154 patients who were suspected of having respiratory tract infections associated with M. pneumoniae. Results Compared with the results of qPCR, the sensitivity and specificity of the Smart Gene system were 98.7% (78/79) and 100.0% (75/75), respectively. A point mutation at domain V of the 23S rRNA gene was detected from 7 (9.0%) of 78 M. pneumoniae-positive samples by the Smart Gene system and these results were confirmed by direct sequencing. The minimum inhibitory concentrations of clarithromycin among the 5 isolates of M. pneumoniae with a point mutation at domain V of the 23S rRNA gene were >64 mu g/ml and those among the 33 isolates without a mutation in the 23S rRNA gene were Conclusion The Smart Gene system is a rapid and accurate assay for detection of the existence of M. pneumoniae and a point mutation at domain V of the 23S rRNA gene of M. pneumoniae at the same time. The Smart Gene system is suitable for point-of-care testing in both hospital and outpatient settings.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
PISSN	1932-6203
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1371/journal.pone.0258694