open access

https://hdl.handle.net/2115/8401

Up-regulation of P450arom and down-regulation of Dmrt-1 genes in the temperaturedependent sex reversal from genetic males to phenotypic females in a salamander

英語

sex reversal

2006-04

Springer

Development Genes and Evolution

216(4)

224-228

The original publication is available at http://www.springerlink.com/

When larvae of the salamander Hynobius retardatus were reared at a high temperature (28°C) during their thermosensitive period (TSP: from 15 to 30 days after hatching), all larvae developed to phenotypic females irrespective of their genetic sexes. Hynobius P450 aromatase (P450arom) and Dmrt-1 cDNAs were isolated and their expression pattern was analyzed by competitive and conventional RT-PCR. While P450arom gene was expressed predominantly in the ovary, Dmrt-1 was expressed exclusively in the testis. When larvae were reared at the female-producing temperature (28°C) during the TSP, strong expression of the P450arom and complete suppression of the Dmrt-1 genes were induced in all experimental larvae. Up-regulation of the P450arom and down-regulation of Dmrt-1 genes even in genetic males constitute a part of the molecular biological cascade for the temperature-dependent sex reversal from genetic males to phenotypic females in this salamander.

学術雑誌論文

Accepted Manuscript

0949-944X

1432-041X

AA11089872