In vitro evaluation of a cysteine protease from poultry red mites, Demanyssus gallinae, as a vaccine antigen for chickens

Ariizumi, Takuma; Murata, Shiro; Fujisawa, Sotaro; Isezaki, Masayoshi; Sato, Takumi; Oishi, Eiji; Taneno, Akira; Ichii, Osamu; Maekawa, Naoya; Okagawa, Tomohiro; Konnai, Satoru; Ohashi, Kazuhiko

In vitro evaluation of a cysteine protease from poultry red mites, Demanyssus gallinae, as a vaccine antigen for chickens

Ariizumi, Takuma; Murata, Shiro; Fujisawa, Sotaro et al.

Poultry Science, 2022, 101(3), 101638

Permalink : https://hdl.handle.net/2115/84051

このアイテムのアクセス数:436件（2026-01-17 00:22 集計）

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アクセス権	metadata only access
URI	https://hdl.handle.net/2115/84051
タイトル	In vitro evaluation of a cysteine protease from poultry red mites, Demanyssus gallinae, as a vaccine antigen for chickens
著者	Ariizumi, Takuma ; Murata, Shiro ; Fujisawa, Sotaro ; Isezaki, Masayoshi ; Sato, Takumi ; Oishi, Eiji ; Taneno, Akira ; Ichii, Osamu ; Maekawa, Naoya ; Okagawa, Tomohiro ; Konnai, Satoru ; Ohashi, Kazuhiko
著者名 Ariizumi, Takuma
e-Rad_Researcher 10579163 著者名 Murata, Shiro
著者名 Fujisawa, Sotaro
著者名 Isezaki, Masayoshi
著者名 Sato, Takumi
著者名 Oishi, Eiji
著者名 Taneno, Akira
e-Rad_Researcher 60547769 著者名 Ichii, Osamu
e-Rad_Researcher 70829035 著者名 Maekawa, Naoya
e-Rad_Researcher 80829036 著者名 Okagawa, Tomohiro
e-Rad_Researcher 40396304 著者名 Konnai, Satoru
e-Rad_Researcher 90250498 著者名 Ohashi, Kazuhiko
言語	英語
キーワード	poultry red mite
	cysteine protease
	vaccine candidate
	Deg-CPR-2
	Dermanyssus gallinae
発行日	2022-03
出版者	Elsevier
収録物名	Poultry Science
巻(号)	101(3)
ページ	101638
抄録	Poultry red mites (PRMs, Dermanyssus gallinae) are hematophagous ectoparasites that negatively affect egg production, which causes serious economic losses to the poultry industry worldwide. Currently, the emergence of acaricide-resistant PRMs has impeded PRM control in poultry farms. Several alternatives for acaricide use have been described for managing PRM-caused problems. Vaccination is among the methods for controlling PRMs in poultry houses. Currently, several candidates for vaccine antigens have been identified. This study identified a cysteine protease, Deg-CPR-2, which differs from 2 other previously reported cysteine proteases in PRMs, from previously obtained data from RNA-sequencing (RNA-seq) analysis. We investigated the characteristics of Deg-CPR-2 and assessed its efficacy as a vaccine antigen in vitro. Phylogenetic analysis revealed that Deg-CPR-2 belonged to a different cluster from those of other cysteine proteases in PRMs. This cluster also included cathepsin L-like proteases, enzymes thought to be involved in hemoglobin digestion in ticks. Expression analysis revealed Deg-CPR-2 expression in midguts and all the life-stages; however, there were differences in the expression levels across the life-stages. The enzyme activity of recombinant Deg-CPR-2 was inhibited in the presence of a cysteine protease inhibitor, which suggests that Deg-CPR2 functions as a cysteine protease in PRMs. Finally, there was an in vitro increase in the mortality of PRMs, mainly protonymphs that were artificially fed with plasma from chickens immunized with Deg-CPR-2. These findings suggest that Deg-CPR-2 may contribute to protein digestion in the midgut of PRMs and is crucially involved in physiological processes in PRMs. Additionally, immunization with Deg-CPR-2 may reduce the number of protonymphs, and Deg-CPR-2 should be considered as a candidate antigen for anti-PRM vaccine development.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1016/j.psj.2021.101638