Structural Requirements in the Hemagglutinin Cleavage Site-Coding RNA Region for the Generation of Highly Pathogenic Avian Influenza Virus

Kida, Yurie; Okuya, Kosuke; Saito, Takeshi; Yamagishi, Junya; Ohnuma, Aiko; Hattori, Takanari; Miyamoto, Hiroko; Manzoor, Rashid; Yoshida, Reiko; Nao, Naganori; Kajihara, Masahiro; Watanabe, Tokiko; Takada, Ayato

Structural Requirements in the Hemagglutinin Cleavage Site-Coding RNA Region for the Generation of Highly Pathogenic Avian Influenza Virus

Kida, Yurie; Okuya, Kosuke; Saito, Takeshi et al.

Pathogens, 2021, 10(12), 1597

Permalink : https://hdl.handle.net/2115/84077

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アクセス権	metadata only access
URI	https://hdl.handle.net/2115/84077
タイトル	Structural Requirements in the Hemagglutinin Cleavage Site-Coding RNA Region for the Generation of Highly Pathogenic Avian Influenza Virus
著者	Kida, Yurie ; Okuya, Kosuke ; Saito, Takeshi ; Yamagishi, Junya ; Ohnuma, Aiko ; Hattori, Takanari ; Miyamoto, Hiroko ; Manzoor, Rashid ; Yoshida, Reiko ; Nao, Naganori ; Kajihara, Masahiro ; Watanabe, Tokiko ; Takada, Ayato
著者名 Kida, Yurie
e-Rad_Researcher 10907736 著者名 Okuya, Kosuke
e-Rad_Researcher 40153811 著者名 Saito, Takeshi
e-Rad_Researcher 80535328 著者名 Yamagishi, Junya
著者名 Ohnuma, Aiko
著者名 Hattori, Takanari
著者名 Miyamoto, Hiroko
著者名 Manzoor, Rashid
e-Rad_Researcher 80435966 著者名 Yoshida, Reiko
e-Rad_Researcher 00781741 著者名 Nao, Naganori
e-Rad_Researcher 70711894 著者名 Kajihara, Masahiro
e-Rad_Researcher 60557479 著者名 Watanabe, Tokiko
e-Rad_Researcher 10292062 著者名 Takada, Ayato
言語	英語
キーワード	influenza virus
	highly pathogenic avian influenza virus
	hemagglutinin
	cleavage site
	RNA secondary structure
	nucleotide insertion
発行日	2021-12
出版者	MDPI
収録物名	Pathogens
巻(号)	10(12)
ページ	1597
抄録	Highly pathogenic avian influenza viruses (HPAIVs) with H5 and H7 hemagglutinin (HA) subtypes are derived from their low pathogenic counterparts following the acquisition of multiple basic amino acids in their HA cleavage site. It has been suggested that consecutive adenine residues and a stem-loop structure in the viral RNA region that encodes the cleavage site are essential for the acquisition of the polybasic cleavage site. By using a reporter assay to detect non-templated nucleotide insertions, we found that insertions more frequently occurred in the RNA region (29 nucleotide-length) encoding the cleavage site of an H5 HA gene that was predicted to have a stem-loop structure containing consecutive adenines than in a mutated corresponding RNA region that had a disrupted loop structure with fewer adenines. In virus particles generated by using reverse genetics, nucleotide insertions that created additional codons for basic amino acids were found in the RNA region encoding the cleavage site of an H5 HA gene but not in the mutated RNA region. We confirmed the presence of virus clones with the ability to replicate without trypsin in a plaque assay and to cause lethal infection in chicks. These results demonstrate that the stem-loop structure containing consecutive adenines in HA genes is a key molecular determinant for the emergence of H5 HPAIVs.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
PISSN	2076-0817
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.3390/pathogens10121597