Molecular identification and genetic characterization of tick-borne pathogens in sheep and goats at two farms in the central and southern regions of Malawi

Chatanga, Elisha; Kainga, Henson; Maganga, Emmanuel; Hayashida, Kyoko; Katakura, Ken; Sugimoto, Chihiro; Nonaka, Nariaki; Nakao, Ryo

Molecular identification and genetic characterization of tick-borne pathogens in sheep and goats at two farms in the central and southern regions of Malawi

Chatanga, Elisha; Kainga, Henson; Maganga, Emmanuel et al.

Ticks and Tick-Borne Diseases, 2021, 12(2), 101629

Permalink : https://hdl.handle.net/2115/84251

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アクセス権	open access
URI	https://hdl.handle.net/2115/84251
タイトル	Molecular identification and genetic characterization of tick-borne pathogens in sheep and goats at two farms in the central and southern regions of Malawi
著者	Chatanga, Elisha ; Kainga, Henson ; Maganga, Emmanuel ; Hayashida, Kyoko ; Katakura, Ken ; Sugimoto, Chihiro ; Nonaka, Nariaki ; Nakao, Ryo
著者名 Chatanga, Elisha
著者名 Kainga, Henson
著者名 Maganga, Emmanuel
著者名 Hayashida, Kyoko
e-Rad_Researcher 10130155 著者名 Katakura, Ken
e-Rad_Researcher 90231373 著者名 Sugimoto, Chihiro
e-Rad_Researcher 50281853 著者名 Nonaka, Nariaki
e-Rad_Researcher 50633955 著者名 Nakao, Ryo
言語	英語
キーワード	Tick-borne pathogens
	Goats
	Sheep
	Malawi
	Molecular identification
	Genetic characterization
発行日	2021-03-01
出版者	Elsevier
収録物名	Ticks and Tick-Borne Diseases
巻(号)	12(2)
ページ	101629
抄録	Tick-borne diseases (TBDs) caused by pathogens belonging to the genera Anaplasma, Ehrlichia, Babesia and Theileria in small ruminants are widespread in the tropical and sub-tropical countries. The epidemiology of tick borne pathogens (TBPs) in small ruminants is less understood compared to those infecting cattle in general. This study was carried out to investigate and characterize TBPs in sheep and goats using molecular tools. A total of 107 blood samples from sheep (n = 8) and goats (n = 99) were collected from animals that were apparently healthy from two farms in the central and the southern regions of Malawi. The V4 hypervariable region of the 18S ribosomal RNA gene (rDNA) and the V1 hypervariable region of the 16S rDNA polymerase chain reaction (PCR) assays were used for detection of tick-borne piroplasms and Anaplasmataceae, respectively. Almost the full-length 18S rDNA and the heat shock protein (groEL) gene sequences were used for genetic characterization of the piroplasms and Anaplasmataceae, respectively. The results showed that 76.6 % of the examined animals (n = 107) were positive for at least one TBP. The overall co-infection with at least two TBPs was observed in fortyeight animals (45 %). The detected TBPs were Anaplasma ovis (65 %), Ehrlichia ruminantium (4%), Ehrlichia canis (2%), Babesia strain closely related to Babesia gibsoni (1%), Theileria ovis (52 %), Theileria mutans (3%), Theileria separata (2%), Anaplasma sp. (1%) and Theileria sp. strain MSD-like (17 %). To the authors knowledge this is the first molecular study of TBPs in sheep and goats in Malawi. These results have therefore provided a significant milestone in the knowledge of occurrence of TBPs in sheep and goats in Malawi, which is prerequisite to proper diagnosis and control.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
権利情報	© 2021. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
PISSN	1877-959X
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.ttbdis.2020.101629