Intermittent PTH Administration Increases Bone-Specific Blood Vessels and Surrounding Stromal Cells in Murine Long Bones

Zhao, Shen; Hasegawa, Tomoka; Hongo, Hiromi; Yamamoto, Tomomaya; Abe, Miki; Yoshida, Taiji; Haraguchi, Mai; de Freitas, Paulo Henrique Luiz; Li, Minqi; Tei, Kanchu; Amizuka, Norio

Intermittent PTH Administration Increases Bone-Specific Blood Vessels and Surrounding Stromal Cells in Murine Long Bones

Zhao, Shen; Hasegawa, Tomoka; Hongo, Hiromi et al.

Calcified tissue international, 2021, 108(3), 391-406

Permalink : https://hdl.handle.net/2115/84254

このアイテムのアクセス数:532件（2026-01-23 23:52 集計）

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アクセス権	open access
URI	https://hdl.handle.net/2115/84254
タイトル	Intermittent PTH Administration Increases Bone-Specific Blood Vessels and Surrounding Stromal Cells in Murine Long Bones
著者	Zhao, Shen ; Hasegawa, Tomoka ; Hongo, Hiromi ; Yamamoto, Tomomaya ; Abe, Miki ; Yoshida, Taiji ; Haraguchi, Mai ; de Freitas, Paulo Henrique Luiz ; Li, Minqi ; Tei, Kanchu ; Amizuka, Norio
著者名 Zhao, Shen
e-Rad_Researcher 50739349 著者名 Hasegawa, Tomoka
e-Rad_Researcher 00778970 著者名 Hongo, Hiromi
著者名 Yamamoto, Tomomaya
著者名 Abe, Miki
著者名 Yoshida, Taiji
著者名 Haraguchi, Mai
著者名 de Freitas, Paulo Henrique Luiz
e-Rad_Researcher 60447612 著者名 Li, Minqi
e-Rad_Researcher 80180066 著者名 Tei, Kanchu
e-Rad_Researcher 30242431 著者名 Amizuka, Norio
言語	英語
キーワード	Blood vessel
	Bone
	Parathyroid hormone (PTH)
	Endomucin
	Vascular smooth muscle cell
発行日	2021-03
出版者	Springer
収録物名	Calcified tissue international
巻(号)	108(3)
ページ	391-406
抄録	To verify whether PTH acts on bone-specific blood vessels and on cells surrounding these blood vessels, 6-week-old male mice were subjected to vehicle (control group) or hPTH [1-34] (20 mu g/kg/day, PTH group) injections for 2 weeks. Femoral metaphyses were used for histochemical and immunohistochemical studies. In control metaphyses, endomucin-positive blood vessels were abundant, but alpha SMA-reactive blood vessels were scarce. In the PTH-administered mice, the lumen of endomucin-positive blood vessels was markedly enlarged. Moreover, many alpha SMA-positive cells were evident near the blood vessels, and seemed to derive from those vessels. These alpha SMA-positive cells neighboring the blood vessels showed features of mesenchymal stromal cells, such as immunopositivity for c-kit and tissue nonspecific alkaline phosphatase (TNALP). Thus, PTH administration increased the population of perivascular/stromal cells positive for alpha SMA and c-kit, which were likely committed to the osteoblastic lineage. To understand the cellular events that led to increased numbers and size of bone-specific blood vessels, we performed immunohistochemical studies for PTH/PTHrP receptor and VEGF. After PTH administration, PTH/PTHrP receptor, VEGF and its receptor flk-1 were consistently identified in both osteoblasts and blood vessels (endothelial cells and surrounding perivascular cells). Our findings suggest that exogenous PTH increases the number and size of bone-specific blood vessels while fostering perivascular/stromal cells positive for alpha SMA/TNALP/c-kit.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	This version of the article has been accepted for publication, after peer review (when applicable) and is subject to Springer Nature’s AM terms of use, but is not the Version of Record and does not reflect post-acceptance improvements, or any corrections. The Version of Record is available online at: http://dx.doi.org/10.1007/s00223-020-00776-2
PISSN	0171-967X
関連情報	PMID 33170307
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1007/s00223-020-00776-2