Downregulation of AlphaB-crystallin in Retinal Pigment Epithelial Cells Exposed to Diabetes-related Stimuli In Vivo and In Vitro

Wu, Di; Kase, Satoru; Liu, Ye; Kanda, Atsuhiro; Murata, Miyuki; Ishida, Susumu

Downregulation of AlphaB-crystallin in Retinal Pigment Epithelial Cells Exposed to Diabetes-related Stimuli In Vivo and In Vitro

Wu, Di; Kase, Satoru; Liu, Ye et al.

In vivo, 2022, 36(1), 132-139

Permalink : https://hdl.handle.net/2115/84256

このアイテムのアクセス数:317件（2026-01-19 04:28 集計）

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https://doi.org/10.21873/invivo.12684

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アクセス権	metadata only access
URI	https://hdl.handle.net/2115/84256
タイトル	Downregulation of AlphaB-crystallin in Retinal Pigment Epithelial Cells Exposed to Diabetes-related Stimuli In Vivo and In Vitro
著者	Wu, Di ; Kase, Satoru ; Liu, Ye ; Kanda, Atsuhiro ; Murata, Miyuki ; Ishida, Susumu
著者名 Wu, Di
e-Rad_Researcher 60374394 著者名 Kase, Satoru
著者名 Liu, Ye
著者名 Kanda, Atsuhiro
e-Rad_Researcher 50423752 著者名 Murata, Miyuki
e-Rad_Researcher 10245558 著者名 Ishida, Susumu
言語	英語
キーワード	AlphaB-crystallin
	retinal pigment epithelial cells
	oxidative stress
	high glucose
	streptozotocin
	advanced glycation end-products
発行日	2022-01
出版者	International Institute of Anticancer Research
収録物名	In vivo
巻(号)	36(1)
ページ	132-139
抄録	Background/Aim: AlphaB-crystallin plays a pivotal role in many diseases. However, the involvement of alphaB-crystallin in retinal pigment epithelial (RPE) cells with diabetes stimuli remains unknown. The aim of this study is to examine the alterations of RPE cells and alphaB-crystallin expression in diabetic models in vivo and in vitro. Materials and Methods: Diabetic conditions in mice were induced by streptozotocin (STZ). The thickness of the RPE/choroid complex was measured by optical coherence tomography (OCT). Periodic acid-Schiff (PAS) staining was used to investigate the choriocapillaris in histological sections of murine eyeballs and oxidative stress was evaluated using immunofluorescence with anti-4-hydroxynonenal (HNE) antibody. AlphaB-crystallin expression was examined in the RPE/choroid complex using ELISA. Real-Time PCR was performed to evaluate the alphaB-crystallin expression in cultured human RPE cells with high glucose or following advanced glycation end-products (AGE) stimulation. Results: In diabetic mice, OCT-based RPE/choroidal layers were thickened 2 months after STZ stimulation, where PAS-positive dilated choriocapillaris was noted. Immunoreactivity of 4HNE was strongly observed in the RPE layer, from which a significant number of RPE cells was lost. Meanwhile, alphaBcrystallin expression in 2-month STZ mice was significantly lower compared to controls. In accordance with these results, in vitro data showed that the alphaB-crystallin expression was also significantly lower in RPE cells with high glucose or following AGE stimulation compared to untreated cells. Conclusion: In both types of diabetic models the expression of alphaB-crystallin was found to be downregulated in RPE cells and was associated with increased levels of oxidative stress.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
PISSN	0258-851X
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.21873/invivo.12684