Genetic heterogeneity during breast cancer progression in young patients

Hagio, Kanako; Hatanaka, Kanako C.; Amano, Toraji; Matsuno, Yoshihiro; Hatanaka, Yutaka; Yamashita, Hiroko

Genetic heterogeneity during breast cancer progression in young patients

Hagio, Kanako; Hatanaka, Kanako C.; Amano, Toraji et al.

Breast, 2021, 60, 206-213

Permalink : https://hdl.handle.net/2115/84262

このアイテムのアクセス数:341件（2026-03-15 21:37 集計）

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ファイル	フォーマット	サイズ	閲覧回数	説明
https://doi.org/10.1016/j.breast.2021.10.011			3

論文情報

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アクセス権	metadata only access
URI	https://hdl.handle.net/2115/84262
タイトル	Genetic heterogeneity during breast cancer progression in young patients
著者	Hagio, Kanako ; Hatanaka, Kanako C. ; Amano, Toraji ; Matsuno, Yoshihiro ; Hatanaka, Yutaka ; Yamashita, Hiroko
著者名 Hagio, Kanako
著者名 Hatanaka, Kanako C.
著者名 Amano, Toraji
著者名 Matsuno, Yoshihiro
e-Rad_Researcher 30589924 著者名 Hatanaka, Yutaka
e-Rad_Researcher 70332947 著者名 Yamashita, Hiroko
言語	英語
キーワード	Breast cancer
	Ductal carcinoma in situ
	Invasive carcinoma
	Heterogeneity
	Targeted sequencing
	Young women
発行日	2021-12
出版者	Elsevier
収録物名	Breast
巻(号)	60
ページ	206-213
抄録	Background: Because a number of years may be required for normal cells to develop into carcinoma, genes involved in tumorigenesis and progression might differ among breast cancers in young women and those in older women. The present study sought to analyze subclonality during breast cancer evolution as well as diversity within each individual in our young patients' cohort. Methods: A total of 13 women aged <35 years at diagnosis with early breast cancer were recruited. Serial sections of breast samples consisting of synchronous invasive carcinoma, adjacent ductal carcinoma in situ (DCIS), normal breast tissue, and metastatic lymph nodes were collected and prepared for immunohistochemical analysis of estrogen receptor, progesterone receptor, HER2, and Ki67, and for extraction of genomic DNA. Germline and somatic gene alterations of genomic DNA were examined by targeted sequencing. Results: Genomic DNA from 13 blood samples and 36 breast tissues consisting of 14 invasive carcinomas, nine adjacent DCIS, 11 normal breast tissues, and two metastatic lymph nodes were successfully sequenced. Germline gene alterations including pathogenic variants and gene alterations that were not yet evaluated for their clinical significance were detected in all patients but one. Somatic gene alterations were identified in eight invasive carcinomas, five DCIS, and one metastatic lymph node. Different somatic gene alterations between invasive carcinoma and DCIS were detected in two patients. Somatic gene mutations were present in non-neoplastic tissues in three patients. No two patients had the same gene alterations. Conclusion: Our results reveal diversity within each individual during breast cancer progression. (c) 2021 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
PISSN	0960-9776
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1016/j.breast.2021.10.011