open access

https://hdl.handle.net/2115/84265

Pathological Alternations of Mediastinal Fat-Associated Lymphoid Cluster and Lung in a Streptozotocin-Induced Diabetic Mouse Model

英語

streptozotocin

2021-02

Cambridge University Press

Microscopy and microanalysis

27(1)

187-200

Diabetes is a devastating global health problem and is considered a predisposing factor for lung injury progression. Furthermore, previous reports of the authors revealed the role of mediastinal fat-associated lymphoid clusters (MFALCs) in advancing respiratory diseases. However, no reports concerning the role of MFALCs on the development of lung injury in diabetes have been published. Therefore, this study aimed to examine the correlations between diabetes and the development of MFALCs and the progression of lung injury in a streptozotocin-induced diabetic mouse model. Furthermore, immunohistochemical analysis for immune cells (CD3(+) T-lymphocytes, B220(+) B-lymphocytes, Iba1(+) macrophages, and Gr1(+) granulocytes), vessels markers (CD31(+) endothelial cells and LYVE-1(+) lymphatic vessels "LVs"), and inflammatory markers (TNF-alpha and IL-5) was performed. In comparison to the control group, the diabetic group showed lung injury development with a significant increase in MFALC size, immune cells, LVs, and inflammatory marker, and a considerable decrease of CD31(+) endothelial cells in both lung and MFALCs was observed. Furthermore, the blood glucose level showed significant positive correlations with MFALCs size, lung injury, immune cells, inflammatory markers, and LYVE-1(+) LVs in lungs and MFALCs. Thus, we suggest that the development of MFALCs and LVs could contribute to lung injury progression in diabetic conditions.

学術雑誌論文

Accepted Manuscript

Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International

This article has been published in a revised form in [Microscopy and Microanalysis] [http://doi.org/10.1017/S1431927620024824]. This version is published under a Creative Commons CC-BY-NC-ND. No commercial re-distribution or re-use allowed. Derivative works cannot be distributed. ©[Elewa, Y., Ichii, O., Nakamura, T., & Kon, Y. ].

1431-9276