Simultaneous steroids measurement in dogs with hyperadrenocorticism using a column-switching liquid chromatography-tandem mass spectrometry method

Sasaki, Noboru; Oo, Thandar; Yasuda, Yoshikazu; Ichise, Takahiro; Nagata, Noriyuki; Yokoyama, Nozomu; Sasaoka, Kazuyoshi; Morishita, Keitaro; Nakayama, Shouta Mm; Ishizuka, Mayumi; Nakamura, Kensuke; Takiguchi, Mitsuyoshi; Ikenaka, Yoshinori

Simultaneous steroids measurement in dogs with hyperadrenocorticism using a column-switching liquid chromatography-tandem mass spectrometry method

Sasaki, Noboru; Oo, Thandar; Yasuda, Yoshikazu et al.

Journal of veterinary medical science, 2021, 83(11), 1634-1642

Permalink : https://hdl.handle.net/2115/84327

このアイテムのアクセス数:358件（2026-01-23 08:09 集計）

閲覧可能ファイル

ファイル	フォーマット	サイズ	閲覧回数	説明
https://doi.org/10.1292/jvms.21-0406

論文情報

ファイル出力 EndNote Basic出力 Mendeley出力

アクセス権	metadata only access
URI	https://hdl.handle.net/2115/84327
タイトル	Simultaneous steroids measurement in dogs with hyperadrenocorticism using a column-switching liquid chromatography-tandem mass spectrometry method
著者	Sasaki, Noboru ; Oo, Thandar ; Yasuda, Yoshikazu ; Ichise, Takahiro ; Nagata, Noriyuki ; Yokoyama, Nozomu ; Sasaoka, Kazuyoshi ; Morishita, Keitaro ; Nakayama, Shouta Mm ; Ishizuka, Mayumi ; Nakamura, Kensuke ; Takiguchi, Mitsuyoshi ; Ikenaka, Yoshinori
e-Rad_Researcher 00754532 著者名 Sasaki, Noboru
著者名 Oo, Thandar
著者名 Yasuda, Yoshikazu
著者名 Ichise, Takahiro
著者名 Nagata, Noriyuki
著者名 Yokoyama, Nozomu
e-Rad_Researcher 40846060 著者名 Sasaoka, Kazuyoshi
e-Rad_Researcher 30637046 著者名 Morishita, Keitaro
著者名 Nakayama, Shouta Mm
e-Rad_Researcher 50332474 著者名 Ishizuka, Mayumi
e-Rad_Researcher 80625898 著者名 Nakamura, Kensuke
e-Rad_Researcher 70261336 著者名 Takiguchi, Mitsuyoshi
e-Rad_Researcher 40543509 著者名 Ikenaka, Yoshinori
言語	英語
キーワード	column
	switching
	dog
	hyperadrenocorticism
	liquid chromatography with triple quadrupole mass spectrometry (LC/MS/MS)
	steroid profile
発行日	2021-10-31
出版者	公益社団法人日本獣医学会 (The Japanese Society of Veterinary Science)
収録物名	Journal of veterinary medical science
巻(号)	83(11)
ページ	1634-1642
抄録	We developed an analytical method using an on-line column-switching liquid chromatography with triple quadrupole mass spectrometry (LC/MS/MS) for quantifying multiple steroids in serum. Using the developed method, we evaluated the serum concentration of nine steroids (cortisol, corticosterone, cortisone, 11-deoxycortisol, 21-deoxycortisol, deoxycorticosterone, progesterone, 17 alpha-OH-progesterone and aldosterone) in dogs with hyperadrenocorticism ( HAC). Serum was mixed with stable isotope internal standards and thereafter purified by the automated column-switching system. The limit of detection ranged 2-16 pg/ml for nine steroids. In the baseline samples, five steroids (cortisol, corticosterone, cortisone, 11-deoxycortisol, and 17 alpha-OH-progesterone) were detected in all dogs. The concentrations of cortisone, 11-deoxycortisol, and 17 alpha-OH-progesterone in dogs with HAC (n=19) were significantly higher those in dogs without HAC (n=15, P<0.02). After the adrenocorticotropic hormone stimulation test, six steroids (cortisol, corticosterone, cortisone, 11-deoxycortisol, 17 alpha-OH- progesterone, and deoxycorticosterone) were above the limit of quantification in all dogs. Cortisol, corticosterone, cortisone, and deoxycorticosterone concentrations of dogs with HAC were significantly higher than those of dogs without HAC (P<0.02). In addition, 11-deoxycortisol and 17 alpha-OH-progesterone concentration was higher in dogs with HAC than in dogs without HAC (P=0.044 and P=0.048, respectively). The on-line column-switching LC/MS/MS would be feasible for measuring multiple steroids in dog serum. The results suggest that cortisone, 11-deoxycortisol, and 17 alpha-OH-progesterone would be related to HAC. Further studies are warranted to assess the clinical feasibility of steroid profile in dogs with HAC.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
PISSN	0916-7250
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1292/jvms.21-0406