Growth Promotion of Giant Duckweed Spirodela polyrhiza (Lemnaceae) by Ensifer sp. SP4 Through Enhancement of Nitrogen Metabolism and Photosynthesis

Toyama, Tadashi; Mori, Kazuhiro; Tanaka, Yasuhiro; Ike, Michihiko; Morikawa, Masaaki

Growth Promotion of Giant Duckweed Spirodela polyrhiza (Lemnaceae) by Ensifer sp. SP4 Through Enhancement of Nitrogen Metabolism and Photosynthesis

Toyama, Tadashi; Mori, Kazuhiro; Tanaka, Yasuhiro et al.

Molecular Plant-Microbe Interactions, 2022, 35(1), 28-38

Permalink : https://hdl.handle.net/2115/84392

このアイテムのアクセス数:525件（2026-03-04 20:34 集計）

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アクセス権	metadata only access
URI	https://hdl.handle.net/2115/84392
タイトル	Growth Promotion of Giant Duckweed Spirodela polyrhiza (Lemnaceae) by Ensifer sp. SP4 Through Enhancement of Nitrogen Metabolism and Photosynthesis
著者	Toyama, Tadashi ; Mori, Kazuhiro ; Tanaka, Yasuhiro ; Ike, Michihiko ; Morikawa, Masaaki
e-Rad_Researcher 60431392 著者名 Toyama, Tadashi
e-Rad_Researcher 90294040 著者名 Mori, Kazuhiro
e-Rad_Researcher 50377587 著者名 Tanaka, Yasuhiro
e-Rad_Researcher 40222856 著者名 Ike, Michihiko
e-Rad_Researcher 20230104 著者名 Morikawa, Masaaki
言語	英語
キーワード	duckweed
	Ensifer sp.
	nitrogen metabolism
	photosynthesis
	plant-bacteria interaction
	plant-growth-promoting bacteria
	Spirodela polyrhiza
発行日	2022-01
出版者	American Phytopathological Society
収録物名	Molecular Plant-Microbe Interactions
巻(号)	35(1)
ページ	28-38
抄録	Duckweeds (Lemnaceae) are representative producers in fresh aquatic ecosystems and also yield sustainable biomass for animal feeds, human foods, and biofuels, and contribute toward effective wastewater treatment; thus, enhancing duckweed productivity is a critical challenge. Plant-growth -promoting bacteria (PGPB) can improve the productivity of terrestrial plants; however, duckweed-PGPB interactions remain unclear and no previous study has investigated the molecular mechanisms underlying duckweed-PGPB interaction. Herein, a PGPB, Ensifer sp. strain SP4, was newly isolated from giant duckweed (Spirodela polyrhiza), and the interactions between S. polyrhiza and SP4 were investigated through physiological, biochemical, and metabolomic analyses. In S. polyrhiza and SP4 coculture, SP4 increased the nitrogen (N), chlorophyll, and ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) contents and the photosynthesis rate of S. polyrhiza by 2.5-, 2.5-, 2.7-, and 2.4-fold, respectively. Elevated photosynthesis increased the relative growth rate and biomass productivity of S. polyrhiza by 1.5 and 2.7-fold, respectively. Strain SP4 significantly altered the metabolomic profile of S. polyrhiza, especially its amino acid profile. N stable isotope analysis revealed that organic N compounds were transferred from SP4 to S. polyrhiza. These N compounds, particularly glutamic acid, possibly triggered the increase in photosynthetic and growth activities. Accordingly, we propose a new model for the molecular mechanism underlying S. polyrhiza growth promotion by its associated bacteria Ensifer sp. SP4, which occurs through enhanced N compound metabolism and photosynthesis. Our findings show that Ensifer sp. SP4 is a promising PGPB for increasing biomass yield, wastewater purification activity, and CO2 capture of S. polyrhiza.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
PISSN	0894-0282
EISSN	1943-7706
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1094/MPMI-06-21-0157-R