Lysophosphatidylethanolamine Affects Lipid Accumulation and Metabolism in a Human Liver-Derived Cell Line

Yamamoto, Yusuke; Sakurai, Toshihiro; Chen, Zhen; Inoue, Nao; Chiba, Hitoshi; Hui, Shu-Ping

Lysophosphatidylethanolamine Affects Lipid Accumulation and Metabolism in a Human Liver-Derived Cell Line

Yamamoto, Yusuke; Sakurai, Toshihiro; Chen, Zhen et al.

Nutrients, 2022, 14(3), 579

Permalink : https://hdl.handle.net/2115/84449

このアイテムのアクセス数:493件（2026-02-10 12:15 集計）

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アクセス権	metadata only access
URI	https://hdl.handle.net/2115/84449
タイトル	Lysophosphatidylethanolamine Affects Lipid Accumulation and Metabolism in a Human Liver-Derived Cell Line
著者	Yamamoto, Yusuke ; Sakurai, Toshihiro ; Chen, Zhen ; Inoue, Nao ; Chiba, Hitoshi ; Hui, Shu-Ping
著者名 Yamamoto, Yusuke
e-Rad_Researcher 60707602 著者名 Sakurai, Toshihiro
e-Rad_Researcher 60802634 著者名 Chen, Zhen
著者名 Inoue, Nao
著者名 Chiba, Hitoshi
e-Rad_Researcher 90337030 著者名 Hui, Shu-Ping
言語	英語
キーワード	lipid droplets
	LC-MS
	MS
	triacylglycerol
	adipose tissue triglyceride lipase
	catabolism
発行日	2022-02
出版者	MDPI
収録物名	Nutrients
巻(号)	14(3)
ページ	579
抄録	The physiological functions of lysophosphatidylethanolamine (lysoPE) have not been fully elucidated. In this study, the effects of lysoPE on lipogenesis and lipolysis were investigated in a cultured human liver-derived cell line. The intracellular lipid profile was investigated in detail using liquid chromatography-tandem mass spectrometry (LC-MS/MS) to better understand the underlying mechanism. The expression of genes related to lipid metabolism and catabolism was analyzed using real-time PCR. LysoPE supplementation induced cellular lipid droplet formation and altered triacylglycerol (TAG) profiles. Furthermore, lysoPE downregulated expression of the TAG hydrolyzation regulation factor ATGL, and reduced the expression of fatty acid biosynthesis-related genes SREBP1 and SCD1. LC-MS/MS-based lipidomic profiling revealed that the addition of lysoPE 18:2 increased the PE species containing linoleic acyl, as well as the CE 18:2 species, likely due to the incorporation of linoleic acyl from lysoPE 18:2. Collectively, these findings suggest that lysoPE 18:2 is involved in lipid droplet formation by suppressing lipolysis and fatty acid biosynthesis. Thus, lysoPE might play a pathological role in the induction of fatty liver disease.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
EISSN	2072-6643
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.3390/nu14030579