RNase in the saliva can affect the detection of severe acute respiratory syndrome coronavirus 2 by real-time one-step polymerase chain reaction using saliva samples

Nishibata, Yuka; Koshimoto, Shota; Ogaki, Kenta; Ishikawa, Erika; Wada, Kosuke; Yoshinari, Miku; Tamura, Yuto; Uozumi, Ryo; Masuda, Sakiko; Tomaru, Utano; Ishizu, Akihiro

RNase in the saliva can affect the detection of severe acute respiratory syndrome coronavirus 2 by real-time one-step polymerase chain reaction using saliva samples

Nishibata, Yuka; Koshimoto, Shota; Ogaki, Kenta et al.

Pathology : Research and Practice, 2021, 220, 153381

Permalink : https://hdl.handle.net/2115/84659

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アクセス権	open access
URI	https://hdl.handle.net/2115/84659
タイトル	RNase in the saliva can affect the detection of severe acute respiratory syndrome coronavirus 2 by real-time one-step polymerase chain reaction using saliva samples
著者	Nishibata, Yuka ; Koshimoto, Shota ; Ogaki, Kenta ; Ishikawa, Erika ; Wada, Kosuke ; Yoshinari, Miku ; Tamura, Yuto ; Uozumi, Ryo ; Masuda, Sakiko ; Tomaru, Utano ; Ishizu, Akihiro
著者名 Nishibata, Yuka
著者名 Koshimoto, Shota
著者名 Ogaki, Kenta
著者名 Ishikawa, Erika
著者名 Wada, Kosuke
著者名 Yoshinari, Miku
著者名 Tamura, Yuto
著者名 Uozumi, Ryo
e-Rad_Researcher 10763617 著者名 Masuda, Sakiko
e-Rad_Researcher 20360901 著者名 Tomaru, Utano
e-Rad_Researcher 60321957 著者名 Ishizu, Akihiro
言語	英語
キーワード	SARS-CoV-2
	COVID-19
	Saliva
	PCR
	RNase
発行日	2021-02-16
出版者	Elsevier
収録物名	Pathology : Research and Practice
巻(号)	220
ページ	153381
抄録	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a single-stranded RNA virus that causes coronavirus disease 2019, which spread worldwide immediately after the first patient infected with this virus was discovered in Wuhan, China, in December 2019. Currently, polymerase chain reaction (PCR) specimens for the detection of SARS-CoV-2 include saliva, nasopharyngeal swabs, and lower respiratory tract-derived materials such as sputum. Initially, nasopharyngeal swab specimens were applied mainly to the PCR detection of SARS-CoV-2. There was a risk of infection to healthcare workers due to coughing or sneezing by the subjects at the time of sample collection. In contrast, saliva specimens have a low risk of droplet infection and are easy to collect, and their application to PCR testing has been promoted. In this study, we have determined the detection limit of SARS-CoV-2 in saliva samples and examined the effects of storage temperature and storage time of saliva samples on the PCR detection results. As a result, 5 × 103 copies of SARS-CoV-2 could be detected in 1 mL phosphate-buffered saline, whereas 5 × 104 copies of SARS-CoV-2 were needed in 1 mL saliva to detect the virus by real-time one-step PCR. Interestingly, SARS-CoV-2 (5 × 103 copies/mL) could be detected in saliva supplemented with an RNase inhibitor. Concerning the saliva samples supplemented with an RNase inhibitor, the optimal temperature for sample storage was −20 °C, and PCR detection was maintained within 48 h without problems under these conditions. These finding suggest that RNase in the saliva can affect the detection of SARS-CoV-2 by PCR using saliva samples.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
権利情報	© 2021. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
PISSN	0344-0338
関連情報	PMID 33640711
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.prp.2021.153381