open access

https://doi.org/10.14943/doctoral.k13862

https://hdl.handle.net/2115/84700

前駆体を利用した生体模倣プロセスにより作製されたアパタイト被覆コラーゲンスキャフォールドの骨形成能の評価

英語

RT-PCR

2020-03-25

Hokkaido University

36p

Three-dimensional collagen scaffold (CS) coated with low-crystalline apatite via a precursor-assisted biomimetic process reportedly enhanced cellular responses and blood vessels formation. In this study, Osteogenic properties of apatite-coated collagen scaffold (Ap-CS) were examined by in vitro cell culture tests and rat bone forming tests. After oxygen plasma treatment, CS was alternately dipped in CaCl2 and K2HPO4・3H2O solutions to be pre-coated with CaP. Subsequently, the CS was immersed in a supersaturated CaP solution to be coated with apatite. The resulting Ap-CS was characterized by observation using a scanning electron microscope (SEM), and assessments of water absorption, Ca ion release, protein adsorption and enzyme resistance. Cytotoxic tests and real-time RT-PCR were carried out using MC3T3-E1 osteoblastic 2cells. In addition, bone forming ability of Ap-CS was histologically evaluated after implantation onto the rat skull.SEM observation revealed that the surface of Ap-CS was covered with nanostructured deposition. It is considered that a layer of low-crystalline apatite was formed on CS through the precursor-assisted biomimetic process as reported previously. Ap-CS significantly increased water adsorption, Ca ion release, cationic protein adsorption and resistance to collagenase enzymatic effect, compared with CS. In cell culture studies, Ap-CS decreased the cell proliferation, however, the expression of bone formation markers, such as bone sialoprotein and osteocalcin, was promoted compared with CS. Ap-CS significantly promoted new bone augmentation of rat skull. Furthermore, residual scaffold was slight when compared to CS. In conclusion, Ap-CS exhibited bone forming activity to be beneficial for bone tissue engineering therapy.

2020-03-25

甲第13862号

博士(歯学)

(主査) 教授 菅谷 勉, 教授 網塚 憲生, 教授 吉田 靖弘

歯学研究科（口腔医学専攻）

博士論文

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