Effect of Insertion and Deletion in the Meq Protein Encoded by Highly Oncogenic Marek's Disease Virus on Transactivation Activity and Virulence

Sato, Jumpei; Murata, Shiro; Yang, Zhiyuan; Kaufer, Benedikt B.; Fujisawa, Sotaro; Seo, Hikari; Maekawa, Naoya; Okagawa, Tomohiro; Konnai, Satoru; Osterrieder, Nikolaus; Parcells, Mark S.; Ohashi, Kazuhiko

Effect of Insertion and Deletion in the Meq Protein Encoded by Highly Oncogenic Marek's Disease Virus on Transactivation Activity and Virulence

Sato, Jumpei; Murata, Shiro; Yang, Zhiyuan et al.

Viruses-Basel, 2022, 14(2), 382-1

Permalink : https://hdl.handle.net/2115/84738

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https://doi.org/10.3390/v14020382

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アクセス権	metadata only access
URI	https://hdl.handle.net/2115/84738
タイトル	Effect of Insertion and Deletion in the Meq Protein Encoded by Highly Oncogenic Marek's Disease Virus on Transactivation Activity and Virulence
著者	Sato, Jumpei ; Murata, Shiro ; Yang, Zhiyuan ; Kaufer, Benedikt B. ; Fujisawa, Sotaro ; Seo, Hikari ; Maekawa, Naoya ; Okagawa, Tomohiro ; Konnai, Satoru ; Osterrieder, Nikolaus ; Parcells, Mark S. ; Ohashi, Kazuhiko
著者名 Sato, Jumpei
e-Rad_Researcher 10579163 著者名 Murata, Shiro
著者名 Yang, Zhiyuan
著者名 Kaufer, Benedikt B.
著者名 Fujisawa, Sotaro
著者名 Seo, Hikari
e-Rad_Researcher 70829035 著者名 Maekawa, Naoya
e-Rad_Researcher 80829036 著者名 Okagawa, Tomohiro
e-Rad_Researcher 40396304 著者名 Konnai, Satoru
著者名 Osterrieder, Nikolaus
著者名 Parcells, Mark S.
e-Rad_Researcher 90250498 著者名 Ohashi, Kazuhiko
言語	英語
キーワード	Marek's disease virus
	Marek's disease
	Meq
	CVI988
	tumorigenesis
	pathogenicity
	transactivation activity
発行日	2022-02
出版者	MDPI
収録物名	Viruses-Basel
巻(号)	14(2)
ページ	382-1
抄録	Marek's disease virus (MDV) causes malignant lymphoma in chickens (Marek's disease, MD). Although MD is currently controlled by vaccination, MDV strains have continuously increased in virulence over the recent decades. Polymorphisms in Meq, an MDV-encoded oncoprotein that serves as a transcription factor, have been associated with the enhanced virulence of the virus. In addition, insertions and deletions in Meq have been observed in MDV strains of higher virulence, but their contribution to said virulence remains elusive. In this study, we investigated the contribution of an insertion (L-Meq) and a deletion in the Meq gene (S-Meq) to its functions and MDV pathogenicity. Reporter assays revealed that both insertion and deletion enhanced the transactivation potential of Meq. Additionally, we generated RB-1B-based recombinant MDVs (rMDVs) encoding each Meq isoform and analyzed their pathogenic potential. rMDV encoding L-Meq indueced the highest mortality and tumor incidence in infected animals, whereas the rMDV encoding S-Meq exhibited the lowest pathogenicity. Thus, insertion enhanced the transactivation activity of Meq and MDV pathogenicity, whereas deletion reduced pathogenicity despite having increased transactivation activity. These data suggest that other functions of Meq affect MDV virulence. These data improve our understanding of the mechanisms underlying the evolution of MDV virulence.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
PISSN	1999-4915
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.3390/v14020382