Altered immunolocalization of FGF23 in murine femora metastasized with human breast carcinoma MDA-MB-231 cells

Yokoyama, Ayako; Hasegawa, Tomoka; Hiraga, Toru; Yamada, Tamaki; Yimin; Hongo, Hiromi; Yamamoto, Tomomaya; Abe, Miki; Yoshida, Taiji; Imanishi, Yasuo; Kuroshima, Shinichiro; Sasaki, Muneteru; de Fraitas, Paulo Henrique Luiz; Li, Minqi; Amizuka, Norio; Yamazaki, Yutaka

Altered immunolocalization of FGF23 in murine femora metastasized with human breast carcinoma MDA-MB-231 cells

Yokoyama, Ayako; Hasegawa, Tomoka; Hiraga, Toru et al.

Journal of Bone and Mineral Metabolism, 2021, 39(5), 810-823

Permalink : https://hdl.handle.net/2115/84740

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アクセス権	open access
URI	https://hdl.handle.net/2115/84740
タイトル	Altered immunolocalization of FGF23 in murine femora metastasized with human breast carcinoma MDA-MB-231 cells
著者	Yokoyama, Ayako ; Hasegawa, Tomoka ; Hiraga, Toru ; Yamada, Tamaki ; Yimin ; Hongo, Hiromi ; Yamamoto, Tomomaya ; Abe, Miki ; Yoshida, Taiji ; Imanishi, Yasuo ; Kuroshima, Shinichiro ; Sasaki, Muneteru ; de Fraitas, Paulo Henrique Luiz ; Li, Minqi ; Amizuka, Norio ; Yamazaki, Yutaka
著者名 Yokoyama, Ayako
e-Rad_Researcher 50739349 著者名 Hasegawa, Tomoka
e-Rad_Researcher 70322170 著者名 Hiraga, Toru
e-Rad_Researcher 80580943 著者名 Yamada, Tamaki
e-Rad_Researcher 40292007 著者名 Yimin
e-Rad_Researcher 00778970 著者名 Hongo, Hiromi
著者名 Yamamoto, Tomomaya
著者名 Abe, Miki
著者名 Yoshida, Taiji
e-Rad_Researcher 50326253 著者名 Imanishi, Yasuo
e-Rad_Researcher 40443915 著者名 Kuroshima, Shinichiro
e-Rad_Researcher 10706336 著者名 Sasaki, Muneteru
著者名 de Fraitas, Paulo Henrique Luiz
著者名 Li, Minqi
e-Rad_Researcher 30242431 著者名 Amizuka, Norio
e-Rad_Researcher 90250464 著者名 Yamazaki, Yutaka
言語	英語
キーワード	Fibroblast growth factor 23
	Osteocyte
	Immunohistochemistry
	Bone metastasis
	MDA-MB-231
発行日	2021-04-08
出版者	Springer
収録物名	Journal of Bone and Mineral Metabolism
巻(号)	39(5)
ページ	810-823
抄録	Introduction After the onset of bone metastasis, tumor cells appear to modify surrounding microenvironments for their benefit, and particularly, the levels of circulating fibroblast growth factor (FGF) 23 in patients with tumors have been highlighted. Materials and methods We have attempted to verify if human breast carcinoma MDA-MB-231 cells metastasized in the long bone of nu/nu mice would synthesize FGF23. Serum concentrations of calcium, phosphate (Pi) and FGF23 were measured in control nu/nu mice, bone-metastasized mice, and mice with mammary gland injected with MDA-MB-231 cells mimicking primary mammary tumors. Results and conclusions MDA-MB-231 cells revealed intense FGF23 reactivity in metastasized lesions, whereas MDA-MB-231 cells cultured in vitro or when injected into the mammary glands (without bone metastasis) showed weak FGF23 immunoreactivity. Although the bone-metastasized MDA-MB-231 cells abundantly synthesized FGF23, osteocytes adjacent to the FGF23-immunopositive tumors, unlike intact osteocytes, showed no FGF23. Despite significantly elevated serum FGF23 levels in bone-metastasized mice, there was no significant decrease in the serum Pi concentration when compared with the intact mice and mice with a mass of MDA-MB-231 cells in mammary glands. The metastasized femora showed increased expression and FGFR1 immunoreactivity in fibroblastic stromal cells, whereas femora of control mice showed no obvious FGFR1 immunoreactivity. Taken together, it seems likely that MDA-MB-231 cells synthesize FGF23 when metastasized to a bone, and thus affect FGFR1-positive stromal cells in the metastasized tumor nest in a paracrine manner.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	This version of the article has been accepted for publication, after peer review (when applicable) and is subject to Springer Nature’s AM terms of use, but is not the Version of Record and does not reflect post-acceptance improvements, or any corrections. The Version of Record is available online at: http://dx.doi.org/10.1007/s00774-021-01220-7
PISSN	0914-8779
NCID	AA10747551
関連情報	PMID 33834310
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1007/s00774-021-01220-7