Amino Acid Substitution Ser83Ile in GyrA of DNA Gyrases Confers High-Level Quinolone Resistance to Nontyphoidal Salmonella Without Loss of Supercoiling Activity

Koide, Kentaro; San, Lai Lai; Pachanon, Ruttana; Park, Jong-Hoon; Ouchi, Yuki; Kongsoi, Siriporn; Utrarachkij, Fuangfa; Nakajima, Chie; Suzuki, Yasuhiko

Amino Acid Substitution Ser83Ile in GyrA of DNA Gyrases Confers High-Level Quinolone Resistance to Nontyphoidal Salmonella Without Loss of Supercoiling Activity

Koide, Kentaro; San, Lai Lai; Pachanon, Ruttana et al.

Microbial drug resistance, 2021, 27(10), 1397-1404

Permalink : https://hdl.handle.net/2115/84983

このアイテムのアクセス数:585件（2026-03-13 17:32 集計）

閲覧可能ファイル

ファイル	フォーマット	サイズ	閲覧回数	説明
Microbial drug resistance_2020_0437	pdf	889 KB	399

論文情報

ファイル出力 EndNote Basic出力 Mendeley出力

アクセス権	open access
URI	https://hdl.handle.net/2115/84983
タイトル	Amino Acid Substitution Ser83Ile in GyrA of DNA Gyrases Confers High-Level Quinolone Resistance to Nontyphoidal Salmonella Without Loss of Supercoiling Activity
著者	Koide, Kentaro ; San, Lai Lai ; Pachanon, Ruttana ; Park, Jong-Hoon ; Ouchi, Yuki ; Kongsoi, Siriporn ; Utrarachkij, Fuangfa ; Nakajima, Chie ; Suzuki, Yasuhiko
著者名 Koide, Kentaro
著者名 San, Lai Lai
著者名 Pachanon, Ruttana
著者名 Park, Jong-Hoon
著者名 Ouchi, Yuki
著者名 Kongsoi, Siriporn
著者名 Utrarachkij, Fuangfa
e-Rad_Researcher 60435964 著者名 Nakajima, Chie
e-Rad_Researcher 90206540 著者名 Suzuki, Yasuhiko
言語	英語
キーワード	antimicrobial resistance
	nontyphoidal Salmonella
	quinolone
	DNA gyrase
発行日	2021-04-20
出版者	Mary Ann Liebert
収録物名	Microbial drug resistance
巻(号)	27(10)
ページ	1397-1404
抄録	Aims: Quinolone-resistant nontyphoidal Salmonella having serine replaced by isoleucine at the 83rd amino acid in GyrA (GyrA-Ser83Ile) has recently been found in Asian countries. In this study, we aimed to examine the direct effect of substitution Ser83Ile on DNA gyrase activity and/or resistance to quinolones. Materials and Methods: Using 50% of the maximal inhibitory concentrations (IC(50)s) of quinolones, recombinant wild type (WT) and seven mutant DNA gyrases having amino acid substitutions, including Ser83Ile, were screened for enzymatic activity that causes supercoils in relaxed plasmid DNA and resistance to quinolones. Results: Little differences in supercoiling activity were observed between WT and mutant DNA gyrases. By contrast, the IC(50)s of ciprofloxacin and norfloxacin against GyrA-Ser83Ile/GyrB-WT were 11.6 and 73.3 mu g/mL, respectively, which were the highest used against the DNA gyrases examined in this study. Conclusion: Ser83Ile in GyrA was shown to confer high-level quinolone resistance to DNA gyrases of nontyphoidal Salmonella, with no loss of supercoiling activity. Salmonella strain carrying GyrA with Ser83Ile may emerge under a high-concentration pressure of quinolones and easily spread even with no selection bias by quinolones. Hence, avoiding the overuse of quinolones is needed to prevent the spread of Salmonella with Ser83Ile in GyrA.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	This is the accepted version of the following article:https://doi.org/10.1089/mdr.2020.0437, which has now been formally published in final form at Microbial Drug Resistance at https://doi.org/10.1089/mdr.2020.0437. This original submission version of the article may be used for non-commercial purposes in accordance with the Mary Ann Liebert, Inc., publishers’ self-archiving terms and conditions.
PISSN	1076-6294
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1089/mdr.2020.0437