Simple and Sensitive Method for the Quantitative Determination of Lipid Hydroperoxides by Liquid Chromatography/Mass Spectrometry

Liang, Chongsheng; Gowda, Siddabasave Gowda B.; Gowda, Divyavani; Sakurai, Toshihiro; Sazaki, Iku; Chiba, Hitoshi; Hui, Shu-Ping

Simple and Sensitive Method for the Quantitative Determination of Lipid Hydroperoxides by Liquid Chromatography/Mass Spectrometry

Liang, Chongsheng; Gowda, Siddabasave Gowda B.; Gowda, Divyavani et al.

Antioxidants, 2022, 11(2), 229

Permalink : https://hdl.handle.net/2115/85070

このアイテムのアクセス数:408件（2026-08-10 08:11 集計）

閲覧可能ファイル

ファイル	フォーマット	サイズ	閲覧回数	説明
https://doi.org/10.3390/antiox11020229			102

論文情報

ファイル出力 EndNote Basic出力 Mendeley出力

アクセス権	open access
URI	https://hdl.handle.net/2115/85070
タイトル	Simple and Sensitive Method for the Quantitative Determination of Lipid Hydroperoxides by Liquid Chromatography/Mass Spectrometry
著者	Liang, Chongsheng ; Gowda, Siddabasave Gowda B. ; Gowda, Divyavani ; Sakurai, Toshihiro ; Sazaki, Iku ; Chiba, Hitoshi ; Hui, Shu-Ping
著者名 Liang, Chongsheng
e-Rad_Researcher 50800504 著者名 Gowda, Siddabasave Gowda B.
著者名 Gowda, Divyavani
e-Rad_Researcher 60707602 著者名 Sakurai, Toshihiro
著者名 Sazaki, Iku
e-Rad_Researcher 70197622 著者名 Chiba, Hitoshi
e-Rad_Researcher 90337030 著者名 Hui, Shu-Ping
言語	英語
キーワード	lipid hydroperoxide
	unsaturated fatty acids
	2-methoxypropene
	chemical derivatization
	liquid chromatography
	mass spectrometry
	human serum
	lipoprotein oxidation
発行日	2022-01-25
出版者	MDPI
収録物名	Antioxidants
巻(号)	11(2)
ページ	229
抄録	Lipid hydroperoxides (LOOH) are the initial products of the peroxidation of unsaturated lipids and play a crucial role in lipid oxidation due to their ability to decompose into free radicals and cause adverse effects on human health. Thus, LOOHs are commonly considered biomarkers of oxidative stress-associated pathological conditions. Despite their importance, the sensitive and selective analytical method for determination is limited, due to their low abundance, poor stability, and low ionizing efficiency. To overcome these limitations, in this study, we chemically synthesized eight fatty acid hydroperoxides (FAOOH), including FA 18:1-OOH, FA 18:2-OOH, FA 18:3-OOH, FA 20:4-OOH, FA 20:5-OOH, FA 22:1-OOH, FA 22:6-OOH as analytes, and FA 19:1-OOH as internal standard. Then, they were chemically labeled with 2-methoxypropene (2-MxP) to obtain FAOOMxP by one-step derivatization (for 10 min). A selected reaction monitoring assisted targeted analytical method was developed using liquid chromatography/tandem mass spectrometry (LC-MS/MS). The MxP-labelling improved the stability and enhanced the ionization efficiency in positive mode. Application of reverse-phase chromatography allowed coelution of analytes and internal standards with a short analysis time of 6 min. The limit of detection and quantification for FAOOH ranged from 0.1-1 pmol/mu L and 1-2.5 pmol/mu L, respectively. The method was applied to profile total FAOOHs in chemically oxidized human serum samples (n = 5) and their fractions of low and high-density lipoproteins (n = 4). The linoleic acid hydroperoxide (FA 18:2-OOH) and oleic acid hydroperoxide (FA 18:1-OOH) were the most abundant FAOOHs in human serum and lipoproteins. Overall, our validated LC-MS/MS methodology features enhanced detection and rapid separation that enables facile quantitation of multiple FAOOHs, therefore providing a valuable tool for determining the level of lipid peroxidation with potential diagnostic applications.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
関連情報	PMID 35204112
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.3390/antiox11020229