Controlling the Rigidity of Kinesin-Propelled Microtubules in an In Vitro Gliding Assay Using the Deep-Sea Osmolyte Trimethylamine N-Oxide

Kabir, Arif Md Rashedul; Munmun, Tasrina; Hayashi, Tomohiko; Yasuda, Satoshi; Kimura, Atsushi P.; Kinoshita, Masahiro; Murata, Takeshi; Sada, Kazuki; Kakugo, Akira

Controlling the Rigidity of Kinesin-Propelled Microtubules in an In Vitro Gliding Assay Using the Deep-Sea Osmolyte Trimethylamine N-Oxide

Kabir, Arif Md Rashedul; Munmun, Tasrina; Hayashi, Tomohiko et al.

ACS Omega, 2022, 7(4), 3796-3803

Permalink : https://hdl.handle.net/2115/85075

このアイテムのアクセス数:390件（2026-01-24 11:11 集計）

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https://doi.org/10.1021/acsomega.1c06699

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アクセス権	metadata only access
URI	https://hdl.handle.net/2115/85075
タイトル	Controlling the Rigidity of Kinesin-Propelled Microtubules in an In Vitro Gliding Assay Using the Deep-Sea Osmolyte Trimethylamine N-Oxide
著者	Kabir, Arif Md Rashedul ; Munmun, Tasrina ; Hayashi, Tomohiko ; Yasuda, Satoshi ; Kimura, Atsushi P. ; Kinoshita, Masahiro ; Murata, Takeshi ; Sada, Kazuki ; Kakugo, Akira
e-Rad_Researcher 10724867 著者名 Kabir, Arif Md Rashedul
著者名 Munmun, Tasrina
著者名 Hayashi, Tomohiko
著者名 Yasuda, Satoshi
e-Rad_Researcher 90422005 著者名 Kimura, Atsushi P.
著者名 Kinoshita, Masahiro
著者名 Murata, Takeshi
e-Rad_Researcher 80225911 著者名 Sada, Kazuki
e-Rad_Researcher 10374224 著者名 Kakugo, Akira
言語	英語
発行日	2022-02-01
出版者	American Chemical Society
収録物名	ACS Omega
巻(号)	7(4)
ページ	3796-3803
抄録	The biomolecular motor protein kinesin and its associated filamentous protein microtubule have been finding important nanotechnological applications in the recent years. Rigidity of the microtubules, which are propelled by kinesin motors in an in vitro gliding assay, is an important metric that determines the success of utilization of microtubules and kinesins in various applications, such as transportation, sensing, sorting, molecular robotics, etc. Therefore, regulating the rigidity of kinesin-propelled microtubules has been critical. In this work, we report a simple strategy to regulate the rigidity of kinesin- propelled microtubules in an in vitro gliding assay. We demonstrate that rigidity of the microtubules, propelled by kinesins in an in nitro gliding assay, can be modulated simply by using the natural osmolyte trimethylamine N-oxide (TMAO). By varying the concentration of TMAO in the gliding assay, the rigidity of microtubules can be modulated over a wide range. Based on this strategy, we are able to reduce the persistence length of microtubules, a measure of microtubule rigidity, similar to 8 fold by using TMAO at the concentration of 1.5 M. Furthermore, we found that the decreased rigidity of the kinesin-propelled microtubules can be restored upon elimination of TMAO from the in nitro gliding assay. Alteration in the rigidity of microtubules is accounted for by the non-uniformity of the force applied by kinesins along the microtubules in the presence of TMAO. This work offers a facile strategy to reversibly regulate the rigidity of kinesin-propelled microtubules in situ, which would widen the applications of the biomolecular motor kinesin and its associated protein microtubule in various fields.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
PISSN	2470-1343
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1021/acsomega.1c06699