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https://hdl.handle.net/2115/85085

N-acetylcysteine attenuates PGE(2) and ROS production stimulated by 4-META/MMA-based resin in murine osteoblastic cells

英語

Reactive oxygen species

2021-05-29

日本歯科理工学会(The Japanese Society for Dental Materials and Devices)

Dental materials journal

40(3)

808-812

This study examined the effects of N-acetylcysteine (NAC) on the inflammatory reactions of murine osteoblastic cells cultured on the 4-methacryloxyethyl trimellitate anhydride/methyl methacrylate (4-META/MMA)-based resin. Superbond C&B (SB) was used as the 4-META/MMA-based resin and placed in a 48-well cell culture plate. The cells were cultured in alpha MEM (control) as well as on SB and SB in alpha MEM with NAC (SB+NAC). They were examined using the WST-1 proliferation assay, real-time PCR, enzyme-linked immunosorbent assay (ELISA), intracellular reactive oxygen species (ROS) measurements, and cellular glutathione (GSH) detection. COX-2 and IL-6 gene expressions were upregulated in SB; however, they were suppressed by NAC. Furthermore, PGE2 production in the culture medium was increased in SB, whereas NAC decreased the PGE(2) production. NAC lowered the ROS level in the culture medium and significantly increased the intracellular GSH level. The present in vitro study demonstrated that NAC might be effective for dental material detoxification.

学術雑誌論文

Version of Record

0287-4547