Changes in Cell Wall Structure During Rhizoid Formation of Silvetia babingtonii (Fucales, Phaeophyceae) Zygotes

Yonamine, Rina; Ichihara, Kensuke; Tsuyuzaki, Shiro; Hervé, Cécile; Motomura, Taizo; Nagasato, Chikako

Changes in Cell Wall Structure During Rhizoid Formation of Silvetia babingtonii (Fucales, Phaeophyceae) Zygotes

Yonamine, Rina; Ichihara, Kensuke; Tsuyuzaki, Shiro et al.

Journal of phycology, 2021, 57(4), 1356-1367

Permalink : https://hdl.handle.net/2115/85093

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論文情報

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アクセス権	open access
URI	https://hdl.handle.net/2115/85093
タイトル	Changes in Cell Wall Structure During Rhizoid Formation of Silvetia babingtonii (Fucales, Phaeophyceae) Zygotes
著者	Yonamine, Rina ; Ichihara, Kensuke ; Tsuyuzaki, Shiro ; Hervé, Cécile ; Motomura, Taizo ; Nagasato, Chikako
著者名 Yonamine, Rina
e-Rad_Researcher 60610095 著者名 Ichihara, Kensuke
e-Rad_Researcher 10222142 著者名 Tsuyuzaki, Shiro
著者名 Hervé, Cécile
e-Rad_Researcher 30183974 著者名 Motomura, Taizo
e-Rad_Researcher 00374710 著者名 Nagasato, Chikako
言語	英語
キーワード	cell wall
	cryofixation
	immunoelectron microscopy
	RNA-seq
	Silvetia babingtonii
	transmission electron microscopy
発行日	2021-05-01
出版者	John Wiley & Sons
収録物名	Journal of phycology
巻(号)	57(4)
ページ	1356-1367
抄録	We examined the ultrastructure of the cell wall and immunolocalization of alginates using specific antibodies against M-rich alginates and MG blocks during rhizoid formation in fucoid zygotes, Silvetia babingtonii. The thallus region of 24-h-old zygotes had a cell wall made of three layers with different fiber distribution. In the 12-h-old zygotes, three layers in the thallus were observed before rhizoid formation, namely the inner, middle, and outer layers. During rhizoid elongation, only the inner layer was apparent close to the rhizoid tip area. Immunoelectron microscopy detected M-rich blocks of alginate on the inner half of the cell wall, irrespective of the number of layers in the thallus and rhizoid regions. The MG blocks were seen to cover a slightly wider area than M-rich alginate blocks. It was suggested that parts of M in mannuronan would be rapidly converted to G, and MG-blocks are generated. Transcriptome analysis was performed using 3 -, 10 -, and 24-h-old zygotes after fertilization to examine the relationship between gene expression and alginate synthesis over time. The expression of two mannuronan C5-epimerase homologs that convert mannuronic acid into guluronic acid in alginates was upregulated or downregulated over the course of the examination.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	This is the peer reviewed version of the following article: Yonamine, R., Ichihara, K., Tsuyuzaki, S., Hervé, C., Motomura, T. and Nagasato, C. (2021), Changes in Cell Wall Structure During Rhizoid Formation of Silvetia babingtonii (Fucales, Phaeophyceae) Zygotes. J. Phycol., which has been published in final form at https://doi.org/10.1111/jpy.13178. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
PISSN	0022-3646
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1111/jpy.13178