Genetic characterization of coliform bacterial isolates from environmental water in Thailand

Tsunoda, Risa; Usui, Masaru; Tagaki, Chie; Fukuda, Akira; Boonla, Chanchai; Anomasiri, Wilai; Sukpanyatham, Nop; Akapelwa, Mwangala Lonah; Nakajima, Chie; Tamura, Yutaka; Suzuki, Yasuhiko

Genetic characterization of coliform bacterial isolates from environmental water in Thailand

Tsunoda, Risa; Usui, Masaru; Tagaki, Chie et al.

Journal of infection and chemotherapy, 2021, 27(5), 722-728

Permalink : https://hdl.handle.net/2115/85103

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アクセス権	open access
URI	https://hdl.handle.net/2115/85103
タイトル	Genetic characterization of coliform bacterial isolates from environmental water in Thailand
著者	Tsunoda, Risa ; Usui, Masaru ; Tagaki, Chie ; Fukuda, Akira ; Boonla, Chanchai ; Anomasiri, Wilai ; Sukpanyatham, Nop ; Akapelwa, Mwangala Lonah ; Nakajima, Chie ; Tamura, Yutaka ; Suzuki, Yasuhiko
著者名 Tsunoda, Risa
著者名 Usui, Masaru
著者名 Tagaki, Chie
著者名 Fukuda, Akira
著者名 Boonla, Chanchai
著者名 Anomasiri, Wilai
著者名 Sukpanyatham, Nop
著者名 Akapelwa, Mwangala Lonah
e-Rad_Researcher 60435964 著者名 Nakajima, Chie
著者名 Tamura, Yutaka
e-Rad_Researcher 90206540 著者名 Suzuki, Yasuhiko
言語	英語
キーワード	Enterobacteriaceae
	Cefazoline-resistant
	IncFrepB plasmid
	bla(TEM)
	tetM
発行日	2021-05-01
出版者	Elsevier
収録物名	Journal of infection and chemotherapy
巻(号)	27(5)
ページ	722-728
抄録	Introduction: In contrast to the study in other part of the world, information about characteristics of plasmids carrying antimicrobial resistance genes (ARGs) in Enterobacteriaceae derived from environmental water in tropical Asian countries including Thailand is limited. This study, therefore, aimed to gain insight into genetic information of antimicrobial resistance in environmental water in Thailand. Methods: Coliform bacteria were isolated from environmental water collected at 20 locations in Thailand and identified. Then, susceptibility profiles to ampicillin, cefazoline, cefotaxime, kanamycin, ciprofloxacin, sulfamethoxazole, tetracycline, and nalidixic acid were assessed. In addition, antimicrobial resistant genes integrons, and replicon types were analyzed. And furthermore, plasmids carrying bla(TEM) and tetM were identified by S1-PFGE analysis and confirmed transmissibility by transconjugation experiments. Results: In 130 coliform bacteria isolated, 89 were resistant to cefazoline while 41 isolates were susceptible. Cefazoline-resistant coliform bacteria were found to be significantly resistant to cefotaxime and tetracycline as compared to susceptible isolates. Hence, blaTEM and tetM correlating with beta-lactam antibiotics and tetracycline, respectively, were analyzed found to co-localize on the IncFrepB plasmids in isolates from pig farms' wastewater by S1-PFGE analysis. And furthermore, transmissibility of the plasmids was confirmed. Conclusions: Results obtained in this study suggested that ARGs in coliform bacteria may have been spreading on the farm via IncFrepB plasmids. Hence, appropriate use of antimicrobials and good hygiene management on the farm are required to prevent the emergence and spread of resistant bacteria. (C) 2021 Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases. Published by Elsevier Ltd. All rights reserved.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
権利情報	© 2021. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/
PISSN	1341-321X
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.jiac.2020.12.023