Stomach-specific calpain, nCL-2, localizes in mucus cells and proteolyzes the β-subunit of coatomer complex, β-COP

Hata, Shoji; Koyama, Suguru; Kawahara, Hiroyuki; Doi, Naoko; Maeda, Tatsuya; Toyama-Sorimachi, Noriko; Abe, Keiko; Suzuki, Koichi; Sorimachi, Hiroyuki

Stomach-specific calpain, nCL-2, localizes in mucus cells and proteolyzes the β-subunit of coatomer complex, β-COP

Hata, Shoji; Koyama, Suguru; Kawahara, Hiroyuki et al.

Journal of biological chemistry, 2006, 281(16), 11214-11224

Permalink : https://hdl.handle.net/2115/8516

このアイテムのアクセス数:1,628件（2026-03-06 08:22 集計）

閲覧可能ファイル

ファイル	フォーマット	サイズ	閲覧回数	説明
Hata et al JBC final	pdf	4.04 MB	1,201

論文情報

ファイル出力 EndNote Basic出力 Mendeley出力

アクセス権	open access
URI	https://hdl.handle.net/2115/8516
タイトル	Stomach-specific calpain, nCL-2, localizes in mucus cells and proteolyzes the β-subunit of coatomer complex, β-COP
著者	Hata, Shoji ; Koyama, Suguru ; Kawahara, Hiroyuki ; Doi, Naoko ; Maeda, Tatsuya ; Toyama-Sorimachi, Noriko ; Abe, Keiko ; Suzuki, Koichi ; Sorimachi, Hiroyuki
著者名 Hata, Shoji
著者名 Koyama, Suguru
著者名 Kawahara, Hiroyuki
著者名 Doi, Naoko
著者名 Maeda, Tatsuya
著者名 Toyama-Sorimachi, Noriko
著者名 Abe, Keiko
著者名 Suzuki, Koichi
著者名 Sorimachi, Hiroyuki
言語	英語
キーワード	tissue-specific caplain
	musscle-specific calpain
	cysteine protease
	crystal-structure
	appendage domain
	saccharomyces-cerevisiae
	active-site
	in-vivo
	gene
発行日	2006-04-21
出版者	AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY
収録物名	Journal of biological chemistry
巻(号)	281(16)
ページ	11214-11224
抄録	Calpain is a Ca2+-regulated cytosolic protease. Mammals have 14 calpain genes, half of which are predominantly expressed in specific organ(s); the rest are expressed ubiquitously. A defect in calpains causes lethality/pathogenicity, indicating their physiological indispensability. nCL-2/calpain-8a was identified as a stomach-specific calpain, whose physiological functions are unclear. To elucidate these, we characterized nCL-2 in detail. Unexpectedly, nCL-2 was localized strictly to the surface mucus cells in the gastric epithelium and the mucus-secreting goblet cells in the duodenum. Yeast two-hybrid screening identified several nCL-2-intracting molecules. Of these, the -subunit of coatomer complex (-COP) occurs in the stomach pit cells and is proteolyzed by nCL-2 in vitro. Furthermore, -COP and nCL-2 co-expressed in COS7 cells co-localized in the Golgi, and Ca2+-ionophore stimulation caused the proteolysis of -COP near the linker region, resulting in the dissociation of -COP from the Golgi. These results strongly suggest novel functions for nCL-2 that involve the membrane trafficking of mucus cells via interactions with coat protein.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	Copyright(c)2006 by the American Society for Biochemistry and Molecular Biology
PISSN	0021-9258
関連情報	URI http://www.jbc.org/
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1074/jbc.M509244200