Characterization of a copper transporter 1 from Dermanyssus gallinae as a vaccine antigen

Fujisawa, Sotaro; Murata, Shiro; Isezaki, Masayoshi; Ariizumi, Takuma; Sato, Takumi; Oishi, Eiji; Taneno, Akira; Maekawa, Naoya; Okagawa, Tomohiro; Ichii, Osamu; Konnai, Satoru; Ohashi, Kazuhiko

Characterization of a copper transporter 1 from Dermanyssus gallinae as a vaccine antigen

Fujisawa, Sotaro; Murata, Shiro; Isezaki, Masayoshi et al.

Parasitology, 2022, 149(1), 105-115

Permalink : https://hdl.handle.net/2115/85161

このアイテムのアクセス数:422件（2026-03-19 18:29 集計）

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https://doi.org/10.1017/S0031182021001608			4

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アクセス権	metadata only access
URI	https://hdl.handle.net/2115/85161
タイトル	Characterization of a copper transporter 1 from Dermanyssus gallinae as a vaccine antigen
著者	Fujisawa, Sotaro ; Murata, Shiro ; Isezaki, Masayoshi ; Ariizumi, Takuma ; Sato, Takumi ; Oishi, Eiji ; Taneno, Akira ; Maekawa, Naoya ; Okagawa, Tomohiro ; Ichii, Osamu ; Konnai, Satoru ; Ohashi, Kazuhiko
著者名 Fujisawa, Sotaro
e-Rad_Researcher 10579163 著者名 Murata, Shiro
著者名 Isezaki, Masayoshi
著者名 Ariizumi, Takuma
著者名 Sato, Takumi
著者名 Oishi, Eiji
著者名 Taneno, Akira
e-Rad_Researcher 70829035 著者名 Maekawa, Naoya
e-Rad_Researcher 80829036 著者名 Okagawa, Tomohiro
e-Rad_Researcher 60547769 著者名 Ichii, Osamu
e-Rad_Researcher 40396304 著者名 Konnai, Satoru
e-Rad_Researcher 90250498 著者名 Ohashi, Kazuhiko
言語	英語
キーワード	Copper transporter 1
	Dermanyssus gallinae
	Dg-Ctr1
	poultry red mite
	vaccine
発行日	2022-01
出版者	Cambridge University Press
収録物名	Parasitology
巻(号)	149(1)
ページ	105-115
抄録	Poultry red mites (Dermanyssus gallinae, PRM) are dangerous ectoparasites that infest chickens and threaten the poultry industry worldwide. PRMs usually develop resistance to chemical acaricides, necessitating the development of more effective preventive agents, and vaccination could be an alternative strategy for controlling PRMs. The suitability of plasma membrane proteins expressed in the midguts as vaccine antigens was evaluated because these molecules are exposed to antibodies in the ingested blood and the binding of antibodies could potentially induce direct damage to midgut tissue and indirect damage via inhibition of the functions of target molecules. Therefore, in the present study, a copper transporter 1-like molecule (Dg-Ctr1) was identified and its efficacy as a vaccine antigen was assessed in vitro. Dg-Ctr1 mRNA was expressed in the midguts and ovaries and in all the life stages, and flow cytometric analysis indicated that Dg-Ctr1 was expressed on the plasma membrane. Importantly, nymphs fed on plasma derived from chickens immunized with the recombinant protein of the extracellular region of Dg-Ctr1 showed a significant reduction in the survival rate. These data indicate that the application of Dg-Ctr1 as a vaccine antigen could reduce the number of nymphs in the farms, contributing to reduction in the economic losses caused by PRMs in the poultry industry. To establish an effective vaccination strategy, the acaricidal effects of the combined use of Dg-Ctr1 with chemical acaricides or other vaccine antigens must be examined.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
PISSN	0031-1820
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1017/S0031182021001608