Mapping of Antibody Epitopes on the Crimean-Congo Hemorrhagic Fever Virus Nucleoprotein

Lombe, Boniface Pongombo; Saito, Takeshi; Miyamoto, Hiroko; Mori-Kajihara, Akina; Kajihara, Masahiro; Saijo, Masayuki; Masumu, Justin; Hattori, Takanari; Igarashi, Manabu; Takada, Ayato

Mapping of Antibody Epitopes on the Crimean-Congo Hemorrhagic Fever Virus Nucleoprotein

Lombe, Boniface Pongombo; Saito, Takeshi; Miyamoto, Hiroko et al.

Viruses-Basel, 2022, 14(3), 544

Permalink : https://hdl.handle.net/2115/85165

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アクセス権	open access
URI	https://hdl.handle.net/2115/85165
タイトル	Mapping of Antibody Epitopes on the Crimean-Congo Hemorrhagic Fever Virus Nucleoprotein
著者	Lombe, Boniface Pongombo ; Saito, Takeshi ; Miyamoto, Hiroko ; Mori-Kajihara, Akina ; Kajihara, Masahiro ; Saijo, Masayuki ; Masumu, Justin ; Hattori, Takanari ; Igarashi, Manabu ; Takada, Ayato
著者名 Lombe, Boniface Pongombo
著者名 Saito, Takeshi
著者名 Miyamoto, Hiroko
著者名 Mori-Kajihara, Akina
著者名 Kajihara, Masahiro
著者名 Saijo, Masayuki
著者名 Masumu, Justin
著者名 Hattori, Takanari
著者名 Igarashi, Manabu
e-Rad_Researcher 10292062 著者名 Takada, Ayato
言語	英語
キーワード	Crimean-Congo hemorrhagic fever virus
	CCHFV
	Nairobi sheep disease virus
	nucleoprotein
	antibody
	epitope
	monoclonal antibody
発行日	2022-03
出版者	MDPI
収録物名	Viruses-Basel
巻(号)	14(3)
ページ	544
抄録	Crimean-Congo hemorrhagic fever virus (CCHFV), a nairovirus, is a tick-borne zoonotic virus that causes hemorrhagic fever in humans. The CCHFV nucleoprotein (NP) is the antigen most used for serological screening of CCHFV infection in animals and humans. To gain insights into antibody epitopes on the NP molecule, we produced recombinant chimeric NPs between CCHFV and Nairobi sheep disease virus (NSDV), which is another nairovirus, and tested rabbit and mouse antisera/immune ascites, anti-NP monoclonal antibodies, and CCHFV-infected animal/human sera for their reactivities to the NP antigens. We found that the amino acids at positions 161-320 might include dominant epitopes recognized by anti-CCHFV IgG antibodies, whereas cross-reactivity between anti-CCHFV and anti-NSDV antibodies was limited. Their binding capacities were further tested using a series of synthetic peptides whose sequences were derived from CCHFV NP. IgG antibodies in CCHFV-infected monkeys and patients were reactive to some of the synthetic peptide antigens (e.g., amino acid residues at positions 131-150 and 211-230). Only a few peptides were recognized by IgG antibodies in the anti-NSDV serum. These results provide useful information to improve NP-based antibody detection assays as well as antigen detection tests relying on anti-NP monoclonal antibodies.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
PISSN	1999-4915
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.3390/v14030544