open access

https://hdl.handle.net/2115/85308

Cholecystokinin secretion induced by β-conglycinin peptone depends on Gαq-mediated pathways in enteroendocrine cells

英語

GPCR

2008

European Journal of Nutrition

48(2)

124-127

Background Intraduodenal administration of peptone prepared from soybean β-conglycinin (BconP) stimulates cholecystokinin (CCK) secretion from enteroendocrine cells, and suppresses food intake in rats. However, the sensing mechanism of BconP by CCK-producing cells is unknown. Aim of the study We investigated signal transduction pathways mediating CCK secretion in response to BconP in the murine CCK-producing cell line, STC-1. Methods STC-1 cells were seeded in 48-well culture plates until sub-confluent and CCK secretion was examined under various conditions. CCK concentration was determined by the enzyme immunoassay. Results BconP dose-dependently induced CCK secretion in STC-1 cells. Treatment with BAPTA-AM, an intracellular Ca2+ chelator, reduced BconP-induced CCK secretion, however, removal of extracellular Ca2+ did not affect the secretory response. Treatment with 2-amino borate (2-APB) reduced CCK releasing responses, suggesting the involvement of IP3. In addition, BconP failed to induce CCK secretion after treatment with the Gαq protein inhibitor (YM-254890). Conclusion These results indicate that Gαq pathway is responsible for BconP-induced CCK secretion in STC-1 cells, and suggest the involvement of a Gαq-coupled GPCR(s) in dietary peptide sensing in enteroendocrine cells.

学術雑誌論文

Accepted Manuscript

The final publication is available at link.springer.com

1436-6207

1436-6215