Soybean β51–63 peptide stimulates cholecystokinin secretion via a calcium-sensing receptor in enteroendocrine STC-1 cells

Nakajima, Shingo; Hira, Tohru; Eto, Yuzuru; Asano, Kozo; Hara, Hiroshi

Soybean β51–63 peptide stimulates cholecystokinin secretion via a calcium-sensing receptor in enteroendocrine STC-1 cells

Nakajima, Shingo; Hira, Tohru; Eto, Yuzuru et al.

Regulatory Peptides, 2010, 159(1-3), 148-155

Permalink : https://hdl.handle.net/2115/85394

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アクセス権	open access
URI	https://hdl.handle.net/2115/85394
タイトル	Soybean β51–63 peptide stimulates cholecystokinin secretion via a calcium-sensing receptor in enteroendocrine STC-1 cells
著者	Nakajima, Shingo ; Hira, Tohru ; Eto, Yuzuru ; Asano, Kozo ; Hara, Hiroshi
著者名 Nakajima, Shingo
e-Rad_Researcher 10396301 著者名 Hira, Tohru
著者名 Eto, Yuzuru
著者名 Asano, Kozo
e-Rad_Researcher 70198894 著者名 Hara, Hiroshi
言語	英語
キーワード	β51–63 peptide
	Enteroendocrine cells
	Calcium-sensing receptor
	Cholecystokinin
発行日	2010-01-08
収録物名	Regulatory Peptides
巻(号)	159(1-3)
ページ	148-155
抄録	We previously demonstrated that intraduodenal administration of an arginine-rich β51-63 peptide in soybean β-conglycinin suppresses food intake via cholecystokinin (CCK) secretion in rats. However, the cellular mechanisms by which the β51-63 peptide induces CCK secretion remain to be clarified. In the present study, we examined whether the extracellular calcium-sensing receptor (CaR) mediates β51-63-induced CCK secretion in murine CCK-producing enteroendocrine cell line STC-1. CCK secretion and changes in intracellular Ca2+ concentration in response to β51-63 peptide were measured in STC-1 cells under various extracellular Ca2+ concentrations and after treatment with a CaR antagonist. Intracellular Ca2+ concentrations in response to β51-63 peptide and extracellular Ca2+ were also measured in CaR-expressing human embryonic kidney (HEK-293) cells. The β51-63 peptide induced CCK secretion and intracellular Ca2+ mobilization in STC-1 cells under normal (1.2 mM) extracellular Ca2+ conditions in a dose-dependent manner. These responses to β51-63 peptide were reduced by the removal of intra- or extracellular Ca2+ but enhanced by increasing extracellular Ca2+ concentrations. Intracellular Ca2+ mobilization induced by extracellular Ca2+ was also increased by the pretreatment with β51-63 peptide. Treatment with a specific CaR antagonist (NPS2143) inhibited β51-63-induced CCK secretion and intracellular Ca2+ mobilization. In addition, HEK-293 cells transfected with CaR acquired sensitivity to the β51-63 peptide. From these results, we conclude that CaR is the β51-63 peptide sensor responsible for the stimulation of CCK secretion in enteroendocrine STC-1 cells.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
権利情報	(c) 2009. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/
PISSN	0167-0115
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.regpep.2009.11.007