FISH Identifies Chromosome Differentiation Between Contemporary Genomes of Wild Types and the Ancestral Genome of Unisexual Clones of Dojo Loach, Misgurnus anguillicaudatus

Kuroda, Masamichi; Shibata, Kiko; Fujimoto, Takafumi et al.

Cytogenetic and genome research, 2021, 161(3-4), 178-186

Permalink : https://hdl.handle.net/2115/85419

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02_FigSet pdf 691 KB 169 Figure
03_SupplementaryTableS1 pdf 131 KB 138 Table
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論文情報

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アクセス権
open access
URI
https://hdl.handle.net/2115/85419
タイトル
FISH Identifies Chromosome Differentiation Between Contemporary Genomes of Wild Types and the Ancestral Genome of Unisexual Clones of Dojo Loach, Misgurnus anguillicaudatus
著者
Kuroda, Masamichi ; Shibata, Kiko ; Fujimoto, Takafumi ; Murakami, Masaru ; Yamaha, Etsuro ; Arai, Katsutoshi
著者名
Kuroda, Masamichi
著者名
Shibata, Kiko
e-Rad_Researcher
10400003
著者名
Fujimoto, Takafumi
e-Rad_Researcher
80271360
著者名
Murakami, Masaru
e-Rad_Researcher
60191376
著者名
Yamaha, Etsuro
e-Rad_Researcher
00137902
著者名
Arai, Katsutoshi
言語
英語
キーワード
Chromosome differentiation
Hybrid
Polyploidy
Unisexual fish
Unreduced gametes
発行日
2021-05-10
出版者
Karger
収録物名
Cytogenetic and genome research
巻(号)
161(3-4)
ページ
178-186
抄録
In dojo loach (Misgurnus anguillicaudatus), although most wild types are gonochoristic diploids that are genetically differentiated into 2 groups, A and B, clonal lineages appear in certain localities. Clonal loaches have been considered to have hybrid origins between the 2 groups by a series of genetic studies. In this study, using FISH with a newly developed probe (ManDra-A), we identified 26 (1 pair of metacentric and 12 pairs of telocentric chromosomes) of 50 diploid chromosomes in contemporary wild-type group A loach. In contrast, ManDra-A signals were not detected on metacentric chromosomes derived from the ancestral group A of clonal loach. The FISH results clearly showed the presence of certain differentiations in metacentric chromosomes between ancestral and contemporary group A loach. Two-color FISH with ManDra-A and group B-specific ManDra (renamed ManDra-B) probes reconfirmed the hybrid origin of clones by identifying chromosomes from both groups A and B in metaphases. Our results showed the hybrid origin of clonally reproducing fish and the possibility that chromosomal differentiation between ancestral and contemporary fish can affect gametogenesis. In meiotic spermatocytes of sex-reversed clones, ManDra-A, and not ManDra-B, signals were detected in 12 out of 50 bivalents. Thus, the results further support the previous conclusion that clonal gametogenesis was assured by pairing between sister chromosomes duplicated from each ancestral chromosome from group A or B. Our study deepens the knowledge about the association between clonality and hybridity in unisexual vertebrates.
資源タイプ
学術雑誌論文
出版タイプ
Accepted Manuscript
権利情報
This is the peer-reviewed but unedited manuscript version of the following article: Cytogenet Genome Res 2021;161:178–186 (DOI:10.1159/000515107). The final, published version is available at https://www.karger.com/?doi=10.1159/000515107
PISSN
1424-8581
EISSN
1424-859X
関連情報 (isVersionOf)
出版社版DOI
https://doi.org/10.1159/000515107