Semen collection by urethral catheterization and electro-ejaculation with different voltages, and the effect of holding temperature and cooling rate before cryopreservation on semen quality in the Japanese macaque (Macaca fuscata)

Takaesu, Noboru; Kanno, Chihiro; Sugimoto, Kosuke; Nagano, Masashi; Kaneko, Akihisa; Indo, Yoriko; Imai, Hiroo; Hirai, Hirohisa; Okamoto, Munehiro; Sashika, Mariko; Shimozuru, Michito; Katagiri, Seiji; Tsubota, Toshio; Yanagawa, Yojiro

Semen collection by urethral catheterization and electro-ejaculation with different voltages, and the effect of holding temperature and cooling rate before cryopreservation on semen quality in the Japanese macaque (Macaca fuscata)

Takaesu, Noboru; Kanno, Chihiro; Sugimoto, Kosuke et al.

Journal of veterinary medical science, 2022, 84(3), 429-438

Permalink : https://hdl.handle.net/2115/85753

このアイテムのアクセス数:386件（2026-01-15 11:55 集計）

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https://doi.org/10.1292/jvms.21-0590			1

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アクセス権	metadata only access
URI	https://hdl.handle.net/2115/85753
タイトル	Semen collection by urethral catheterization and electro-ejaculation with different voltages, and the effect of holding temperature and cooling rate before cryopreservation on semen quality in the Japanese macaque (Macaca fuscata)
著者	Takaesu, Noboru ; Kanno, Chihiro ; Sugimoto, Kosuke ; Nagano, Masashi ; Kaneko, Akihisa ; Indo, Yoriko ; Imai, Hiroo ; Hirai, Hirohisa ; Okamoto, Munehiro ; Sashika, Mariko ; Shimozuru, Michito ; Katagiri, Seiji ; Tsubota, Toshio ; Yanagawa, Yojiro
著者名 Takaesu, Noboru
著者名 Kanno, Chihiro
著者名 Sugimoto, Kosuke
著者名 Nagano, Masashi
著者名 Kaneko, Akihisa
著者名 Indo, Yoriko
著者名 Imai, Hiroo
著者名 Hirai, Hirohisa
著者名 Okamoto, Munehiro
e-Rad_Researcher 30722954 著者名 Sashika, Mariko
e-Rad_Researcher 50507168 著者名 Shimozuru, Michito
e-Rad_Researcher 00292061 著者名 Katagiri, Seiji
e-Rad_Researcher 10207441 著者名 Tsubota, Toshio
e-Rad_Researcher 20609656 著者名 Yanagawa, Yojiro
言語	英語
キーワード	cryopreservation
	electro-ejaculation
	Japanese macaques
	semen collection
	urethral catheterization
発行日	2022-03-15
出版者	公益社団法人日本獣医学会 (The Japanese Society of Veterinary Science)
収録物名	Journal of veterinary medical science
巻(号)	84(3)
ページ	429-438
抄録	In the Japanese macaque, semen has been collected by electro-ejaculation (EE), using the higher voltage stimuli compared to other species including genus Macaca. Semen coagulates immediately after ejaculation, which makes difficult to produce high-quality semen for artificial insemination. Recently, semen collection using urethral catheterization (UC) has been reported in carnivore and this technique may allow semen collection without coagulation in a less invasive manner. Further, the temporal preservation temperature and cooling rate of semen during cryopreservation affect post thawing sperm quality. In this study, to improve semen quality and quantity, as well as the animal welfare, semen collection was performed by EE with high (5-15 V) or low (3-6 V) voltage, UC and a combination of the two (EE-UC). It has been suggested that a high voltage is necessary for semen collection, but 10 V stimulation was effective enough and 15 V is for additional sperm collection. Also, liquid semen was collected by EE-UC and this could increase the total number of sperm. Further, to improve the post thawing sperm motility, semen was kept at four temperatures (4, 15, 25 and 37 degrees C) for 60 min, and processed with two cooling procedures (slow cooling before second dilution and fast cooling after second dilution). Holding semen at 25 degrees C and fast cooling after the second dilution maintained progressive motile sperm rate. The present results will contribute to the improvement of semen collection and animal welfare of Japanese macaques.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
PISSN	0916-7250
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1292/jvms.21-0590