First detection of SARS-CoV-2 RNA in wastewater in North America : A study in Louisiana, USA

Sherchan, Samendra P.; Shahin, Shalina; Ward, Lauren M.; Tandukar, Sarmila; Aw, Tiong G.; Schmitz, Bradley; Ahmed, Warish; Kitajima, Masaaki

First detection of SARS-CoV-2 RNA in wastewater in North America : A study in Louisiana, USA

Sherchan, Samendra P.; Shahin, Shalina; Ward, Lauren M. et al.

Science of the total environment, 2020, 743, 140621

Permalink : https://hdl.handle.net/2115/86171

このアイテムのアクセス数:389件（2026-03-04 17:13 集計）

閲覧可能ファイル

ファイル	フォーマット	サイズ	閲覧回数	説明
STOTEN-D-20-13830R1	pdf	1.46 MB	388

論文情報

ファイル出力 EndNote Basic出力 Mendeley出力

アクセス権	open access
URI	https://hdl.handle.net/2115/86171
タイトル	First detection of SARS-CoV-2 RNA in wastewater in North America : A study in Louisiana, USA
著者	Sherchan, Samendra P. ; Shahin, Shalina ; Ward, Lauren M. ; Tandukar, Sarmila ; Aw, Tiong G. ; Schmitz, Bradley ; Ahmed, Warish ; Kitajima, Masaaki
著者名 Sherchan, Samendra P.
著者名 Shahin, Shalina
著者名 Ward, Lauren M.
著者名 Tandukar, Sarmila
著者名 Aw, Tiong G.
著者名 Schmitz, Bradley
著者名 Ahmed, Warish
著者名 Kitajima, Masaaki
言語	英語
キーワード	Wastewater-based epidemiology
	SARS-CoV-2
	Surveillance
	COVTD-19
	RT-qPCR
	Wastewater
発行日	2020-11-15
出版者	Elsevier
収録物名	Science of the total environment
巻(号)	743
ページ	140621
抄録	We investigated the presence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA in wastewater samples in southern Louisiana, USA. Untreated and treated wastewater samples were collected on five occasions over a four-month period from January to April 2020. The wastewater samples were concentrated via ultrafiltration (Method A). and an adsorption-elution method using electronegative membranes (Method B). SARS-CoV-2 RNA was detected in 2 out of 15 wastewater samples using two reverse transcription-quantitative polymerase chain reaction (RT-qPCR) assays (CDC N1 and N2). None of the secondary treated and final effluent samples tested positive for SARS-CoV-2 RNA. To our knowledge, this is the first study reporting the detection of SAR.S-CoV-2 RNA in wastewater in North America, including the USA. However, concentration methods and RTqPCR assays need to be refined and validated to increase the sensitivity of SARS-CoV-2 RNA detection in wastewater.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
権利情報	© <2020>. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/
PISSN	0048-9697
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.scitotenv.2020.140621