A GCDGC-specific DNA (cytosine-5) methyltransferase that methylates the GCWGC sequence on both strands and the GCSGC sequence on one strand

Furuta, Yoshikazu; Miura, Fumihito; Ichise, Takahiro; Nakayama, Shouta M. M.; Ikenaka, Yoshinori; Zorigt, Tuvshinzaya; Tsujinouchi, Mai; Ishizuka, Mayumi; Ito, Takashi; Higashi, Hideaki

A GCDGC-specific DNA (cytosine-5) methyltransferase that methylates the GCWGC sequence on both strands and the GCSGC sequence on one strand

Furuta, Yoshikazu; Miura, Fumihito; Ichise, Takahiro et al.

PLoS ONE, 2022, 17(3), e0265225

Permalink : https://hdl.handle.net/2115/86223

このアイテムのアクセス数:443件（2026-08-07 21:35 集計）

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論文情報

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アクセス権	open access
URI	https://hdl.handle.net/2115/86223
タイトル	A GCDGC-specific DNA (cytosine-5) methyltransferase that methylates the GCWGC sequence on both strands and the GCSGC sequence on one strand
著者	Furuta, Yoshikazu ; Miura, Fumihito ; Ichise, Takahiro ; Nakayama, Shouta M. M. ; Ikenaka, Yoshinori ; Zorigt, Tuvshinzaya ; Tsujinouchi, Mai ; Ishizuka, Mayumi ; Ito, Takashi ; Higashi, Hideaki
著者名 Furuta, Yoshikazu
著者名 Miura, Fumihito
著者名 Ichise, Takahiro
e-Rad_Researcher 90647629 著者名 Nakayama, Shouta M. M.
e-Rad_Researcher 40543509 著者名 Ikenaka, Yoshinori
著者名 Zorigt, Tuvshinzaya
著者名 Tsujinouchi, Mai
e-Rad_Researcher 50332474 著者名 Ishizuka, Mayumi
著者名 Ito, Takashi
e-Rad_Researcher 20311227 著者名 Higashi, Hideaki
言語	英語
発行日	2022-03-21
出版者	PLOS
収録物名	PLoS ONE
巻(号)	17(3)
ページ	e0265225
抄録	5-Methylcytosine is one of the major epigenetic marks of DNA in living organisms. Some bacterial species possess DNA methyltransferases that modify cytosines on both strands to produce fully-methylated sites or on either strand to produce hemi-methylated sites. In this study, we characterized a DNA methyltransferase that produces two sequences with different methylation patterns: one methylated on both strands and another on one strand. M.BatI is the orphan DNA methyltransferase of Bacillus anthracis coded in one of the prophages on the chromosome. Analysis of M.BatI modified DNA by bisulfite sequencing revealed that the enzyme methylates the first cytosine in sequences of 5ʹ-GCAGC-3ʹ, 5ʹ-GCTGC-3ʹ, and 5ʹ-GCGGC-3ʹ, but not of 5ʹ-GCCGC-3ʹ. This resulted in the production of fully-methylated 5ʹ-GCWGC-3ʹ and hemi-methylated 5ʹ-GCSGC-3ʹ. M.BatI also showed toxicity when expressed in E. coli, which was caused by a mechanism other than DNA modification activity. Homologs of M.BatI were found in other Bacillus species on different prophage like regions, suggesting the spread of the gene by several different phages. The discovery of the DNA methyltransferase with unique modification target specificity suggested unrevealed diversity of target sequences of bacterial cytosine DNA methyltransferase.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution 4.0 International
PISSN	1932-6203
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1371/journal.pone.0265225