1H NMR metabolomic and transcriptomic analyses reveal urinary metabolites as biomarker candidates in response to protein undernutrition in adult rats

Komatsu, Yosuke; Wada, Yasuaki; Izumi, Hirohisa; Shimizu, Takashi; Takeda, Yasuhiro; Aizawa, Tomoyasu

1H NMR metabolomic and transcriptomic analyses reveal urinary metabolites as biomarker candidates in response to protein undernutrition in adult rats

Komatsu, Yosuke; Wada, Yasuaki; Izumi, Hirohisa et al.

British Journal of Nutrition, 2020, 125(6), 633-643

Permalink : https://hdl.handle.net/2115/86311

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論文情報

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アクセス権	open access
URI	https://hdl.handle.net/2115/86311
タイトル	1H NMR metabolomic and transcriptomic analyses reveal urinary metabolites as biomarker candidates in response to protein undernutrition in adult rats
著者	Komatsu, Yosuke ; Wada, Yasuaki ; Izumi, Hirohisa ; Shimizu, Takashi ; Takeda, Yasuhiro ; Aizawa, Tomoyasu
著者名 Komatsu, Yosuke
著者名 Wada, Yasuaki
著者名 Izumi, Hirohisa
著者名 Shimizu, Takashi
著者名 Takeda, Yasuhiro
e-Rad_Researcher 40333596 著者名 Aizawa, Tomoyasu
言語	英語
キーワード	Metabolomics
	Multivariate analysis
	Protein undernutrition
	Transcriptomics
	Urinary metabolites
発行日	2020-08-20
出版者	Cambridge University Press
収録物名	British Journal of Nutrition
巻(号)	125(6)
ページ	633-643
抄録	Protein undernutrition contributes to the development of various diseases in broad generations. Urinary metabolites may serve as non-invasive biomarkers of protein undernutrition; however, this requires further investigation. We aimed to identify novel urinary metabolites as biomarker candidates responsive to protein undernutrition. Adult rats were fed control (CT; 14 % casein) or isoenergetic low-protein (LP; 5 % casein) diets for 4 weeks. 1H NMR metabolomics was applied to urine, plasma and liver samples to identify metabolites responsive to protein undernutrition. Liver samples were subjected to mRNA microarray and quantitative PCR analyses to elucidate the mechanisms causing fluctuations in identified metabolites. Urinary taurine levels were significantly lower in the LP group than in the CT group at week 1 and remained constant until week 4. Hepatic taurine level and gene expression level of cysteine dioxygenase type 1 were also significantly lower in the LP group than in the CT group. Urinary trimethylamine N-oxide (TMAO) levels were significantly higher in the LP group than in the CT group at week 2 and remained constant until week 4. Hepatic TMAO level and gene expression levels of flavin-containing mono-oxygenase 1 and 5 were also significantly higher in the LP group than in the CT group. In conclusion, urinary taurine and TMAO levels substantially responded to protein undernutrition. Furthermore, changes in hepatic levels of these metabolites and gene expressions associated with their metabolic pathways were also reflected in their fluctuating urinary levels. Thus, taurine and TMAO could act as non-invasive urinary biomarker candidates to detect protein undernutrition.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	© The Author(s), 2020. Published by Cambridge University Press on behalf of The Nutrition Society
PISSN	0007-1145
EISSN	1475-2662
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1017/S0007114520003281