Genetic Diversity and Transmission of Multidrug-Resistant Mycobacterium tuberculosis strains in Lusaka, Zambia

Chizimu, Joseph Yamweka; Solo, Eddie Samuneti; Bwalya, Precious et al.

International journal of infectious diseases, 2022, 114, 142-150

Permalink : https://hdl.handle.net/2115/86361

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論文情報

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アクセス権
open access
URI
https://hdl.handle.net/2115/86361
タイトル
Genetic Diversity and Transmission of Multidrug-Resistant Mycobacterium tuberculosis strains in Lusaka, Zambia
著者
Chizimu, Joseph Yamweka ; Solo, Eddie Samuneti ; Bwalya, Precious ; Kapalamula, Thoko Flav ; Akapelwa, Mwangala Lonah ; Lungu, Patrick ; Shrestha, Dipti ; Fukushima, Yukari ; Mukonka, Victor ; Thapa, Jeewan ; Nakajima, Chie ; Suzuki, Yasuhiko
著者名
Chizimu, Joseph Yamweka
著者名
Solo, Eddie Samuneti
著者名
Bwalya, Precious
著者名
Kapalamula, Thoko Flav
著者名
Akapelwa, Mwangala Lonah
著者名
Lungu, Patrick
著者名
Shrestha, Dipti
著者名
Fukushima, Yukari
著者名
Mukonka, Victor
著者名
Thapa, Jeewan
著者名
Nakajima, Chie
e-Rad_Researcher
90206540
著者名
Suzuki, Yasuhiko
言語
英語
キーワード
Mycobacterium tuberculosis
multidrug resistance
mycobacterial interspersed repetitive
units-variable number of tandem repeats (MIRU-VNTR)
Zambia
発行日
2022-01-01
出版者
Elsevier
収録物名
International journal of infectious diseases
巻(号)
114
ページ
142-150
抄録
Objective: Zambia is among the 30 high tuberculosis burden countries in the world. Despite increasing reports of multidrug-resistant tuberculosis (MDR-TB) in routine surveillance, information on the transmission of MDR Mycobacterium tuberculosis strains is largely unknown. This study elucidated the genetic diversity and transmission of MDR M. tuberculosis strains in Lusaka, Zambia. Methods: Eighty-five MDR M. tuberculosis samples collected from 2013 to 2017 at the University Teaching Hospital were used. Drug-resistance associated gene sequencing, spoligotyping, 24-loci mycobacterial interspersed repetitive units-variable number of tandem repeats (MIRU-VNTR), and multiplex PCR for RD Rio sub-lineage identification were applied. Results: The identified clades were LAM (48%), CAS (29%), T (14%), X (6%) and Harlem (2%). Strains belonging to SITs 21/CAS1-Kili and 20/LAM1 formed the largest clonal complexes. Combined spoligotyping and 24 loci-MIRU-VNTR revealed 47 genotypic patterns with a clustering rate of 63%. Ninety-five percent of LAM strains belonged to the RD-Rio sub-lineage. Conclusion: The high clustering rate suggested that a large proportion of MDR-TB was due to recent transmission rather than the independent acquisition of MDR. This spread was attributed to clonal expansion of SIT21/CAS1-Kili and SIT20/LAM1 strains. Therefore, TB control programs recommending genotyping coupled with conventional epidemiological methods can guide measures for stopping the spread of MDR-TB. (C) 2021 The Author(s). Published by Elsevier Ltd on behalf of International Society for Infectious Diseases.
資源タイプ
学術雑誌論文
出版タイプ
Version of Record
PISSN
1201-9712
関連情報 (isIdenticalTo)
出版社版DOI
https://doi.org/10.1016/j.ijid.2021.10.044