Molecular cloning and genetic mapping of perennial ryegrass casein protein kinase 2 α-subunit genes

Shinozuka, H.; Hisano, H.; Ponting, R.C.; Cogan, N.O.I.; Jones, E.S.; Forster, J.W.; Yamada, T.

Molecular cloning and genetic mapping of perennial ryegrass casein protein kinase 2 α-subunit genes

Shinozuka, H.; Hisano, H.; Ponting, R.C. et al.

TAG Theoretical and Applied Genetics, 2005, 112(1), 167-177

Permalink : https://hdl.handle.net/2115/864

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アクセス権	open access
URI	https://hdl.handle.net/2115/864
タイトル	Molecular cloning and genetic mapping of perennial ryegrass casein protein kinase 2 α-subunit genes
著者	Shinozuka, H. ; Hisano, H. ; Ponting, R.C. ; Cogan, N.O.I. ; Jones, E.S. ; Forster, J.W. ; Yamada, T.
著者名 Shinozuka, H.
著者名 Hisano, H.
著者名 Ponting, R.C.
著者名 Cogan, N.O.I.
著者名 Jones, E.S.
著者名 Forster, J.W.
e-Rad_Researcher 70343952 著者名 Yamada, T. 山田, 敏彦
言語	英語
キーワード	Perennial ryegrass
	Casein kinase 2 α
	Single nucleotide polymorphism
	Cleaved amplified polymorphic sequence
	Heading date
	Conserved synteny
発行日	2005-12
出版者	Springer-Verlag GmbH
収録物名	TAG Theoretical and Applied Genetics
巻(号)	112(1)
ページ	167-177
抄録	The α-subunit of the casein protein kinase CK2 has been implicated in both light-regulated and circadian rhythm-controlled plant gene expression, including control of the flowering time. Two putative CK2α genes of perennial ryegrass (Lolium perenne L.) have been obtained from a cDNA library constructed with mRNA isolated from cold-acclimated crown tissue. The genomic organisation of the two genes was determined by Southern hybridisation analysis. Primer designs to the Lpck2a-1 and Lpck2a-2 cDNA sequences permitted the amplification of genomic products containing large intron sequences. Amplicon sequence analysis detected single nucleotide polymorphisms (SNPs) within the p150/112 reference mapping population. Validated SNPs, within diagnostic restriction enzyme sites, were used to design cleaved amplified polymorphic sequence (CAPS) assays. The Lpck2a-1 CAPS marker was assigned to perennial ryegrass linkage group (LG) 4 and the Lpck2a-2 CAPS marker was assigned to LG2. The location of the Lpck2a-1 gene locus supports the previous conclusion of conserved synteny between perennial ryegrass LG4, the Triticeae homoeologous group 5L chromosomes and the corresponding segment of rice chromosome 3. Allelic variation at the Lpck2a-1 and Lpck2a-2 gene loci was correlated with phenotypic variation for heading date and winter survival, respectively. SNP polymorphism may be used for the further study of the role of CK2α genes in the initiation of reproductive development and winter hardiness in grasses.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	The original publication is available at www.springerlink.com
PISSN	0040-5752
関連情報	PMID 16240106
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1007/s00122-005-0119-8