Retrograde Axonal Transport of Liposomes from Peripheral Tissue to Spinal Cord and DRGs by Optimized Phospholipid and CTB Modification

Fukui, Takafumi; Tateno, Hironao; Nakamura, Takashi; Yamada, Yuma; Sato, Yusuke; Iwasaki, Norimasa; Harashima, Hideyoshi; Kadoya, Ken

Retrograde Axonal Transport of Liposomes from Peripheral Tissue to Spinal Cord and DRGs by Optimized Phospholipid and CTB Modification

Fukui, Takafumi; Tateno, Hironao; Nakamura, Takashi et al.

International Journal of Molecular Sciences, 2022, 23(12), 6661

Permalink : https://hdl.handle.net/2115/86520

このアイテムのアクセス数:403件（2026-01-20 00:30 集計）

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https://doi.org/10.3390/ijms23126661

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アクセス権	metadata only access
URI	https://hdl.handle.net/2115/86520
タイトル	Retrograde Axonal Transport of Liposomes from Peripheral Tissue to Spinal Cord and DRGs by Optimized Phospholipid and CTB Modification
著者	Fukui, Takafumi ; Tateno, Hironao ; Nakamura, Takashi ; Yamada, Yuma ; Sato, Yusuke ; Iwasaki, Norimasa ; Harashima, Hideyoshi ; Kadoya, Ken
著者名 Fukui, Takafumi
著者名 Tateno, Hironao
e-Rad_Researcher 20604458 著者名 Nakamura, Takashi
e-Rad_Researcher 60451431 著者名 Yamada, Yuma
e-Rad_Researcher 10735624 著者名 Sato, Yusuke
e-Rad_Researcher 30322803 著者名 Iwasaki, Norimasa
e-Rad_Researcher 00183567 著者名 Harashima, Hideyoshi
e-Rad_Researcher 30374276 著者名 Kadoya, Ken
言語	英語
キーワード	liposome
	drug-delivery system
	retrograde axonal transport
	motor neuron
	DRG
発行日	2022-06-15
出版者	MDPI
収録物名	International Journal of Molecular Sciences
巻(号)	23(12)
ページ	6661
抄録	Despite recent advancements in therapeutic options for disorders of the central nervous system (CNS), the lack of an efficient drug-delivery system (DDS) hampers their clinical application. We hypothesized that liposomes could be optimized for retrograde transport in axons as a DDS from peripheral tissues to the spinal cord and dorsal root ganglia (DRGs). Three types of liposomes consisting of DSPC, DSPC/POPC, or POPC in combination with cholesterol (Chol) and polyethylene glycol (PEG) lipid were administered to sciatic nerves or the tibialis anterior muscle of mature rats. Liposomes in cell bodies were detected with infrared fluorescence of DiD conjugated to liposomes. Three days later, all nerve-administered liposomes were retrogradely transported to the spinal cord and DRGs, whereas only muscle-administered liposomes consisting of DSPC reached the spinal cord and DRGs. Modification with Cholera toxin B subunit improved the transport efficiency of liposomes to the spinal cord and DRGs from 4.5% to 17.3% and from 3.9% to 14.3% via nerve administration, and from 2.6% to 4.8% and from 2.3% to 4.1% via muscle administration, respectively. Modification with octa-arginine (R8) improved the transport efficiency via nerve administration but abolished the transport capability via muscle administration. These findings provide the initial data for the development of a novel DDS targeting the spinal cord and DRGs via peripheral administration.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution 4.0 International
PISSN	1661-6596
EISSN	1422-0067
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.3390/ijms23126661