Novel polychrome staining distinguishing osteochondral tissue and bone cells in decalcified paraffin sections

Nakamura, Teppei; Sumi, Kanako; Tsuji, Erika; Hosotani, Marina; Namba, Takashi; Ichii, Osamu; Irie, Takao; Nagasaki, Ken-ichi; Kon, Yasuhiro; Mishima, Takashi; Yoshiyasu, Tomoji

Novel polychrome staining distinguishing osteochondral tissue and bone cells in decalcified paraffin sections

Nakamura, Teppei; Sumi, Kanako; Tsuji, Erika et al.

Cell and tissue research, 2021, 385, 737

Permalink : https://hdl.handle.net/2115/86586

このアイテムのアクセス数:501件（2026-01-13 18:21 集計）

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アクセス権	open access
URI	https://hdl.handle.net/2115/86586
タイトル	Novel polychrome staining distinguishing osteochondral tissue and bone cells in decalcified paraffin sections
著者	Nakamura, Teppei ; Sumi, Kanako ; Tsuji, Erika ; Hosotani, Marina ; Namba, Takashi ; Ichii, Osamu ; Irie, Takao ; Nagasaki, Ken-ichi ; Kon, Yasuhiro ; Mishima, Takashi ; Yoshiyasu, Tomoji
e-Rad_Researcher 80786773 著者名 Nakamura, Teppei
著者名 Sumi, Kanako
著者名 Tsuji, Erika
著者名 Hosotani, Marina
著者名 Namba, Takashi
e-Rad_Researcher 60547769 著者名 Ichii, Osamu
著者名 Irie, Takao
著者名 Nagasaki, Ken-ichi
e-Rad_Researcher 10178402 著者名 Kon, Yasuhiro
著者名 Mishima, Takashi
著者名 Yoshiyasu, Tomoji
言語	英語
キーワード	Osteoid
	Mineralized bone
	Cartilage
	Bone cells
	Osteochondral staining
発行日	2021-08-19
出版者	Springer
収録物名	Cell and tissue research
巻(号)	385
ページ	737
抄録	The bone is a dynamic and metabolically active organ in which growth and resorption of the osteochondral matrix is orchestrated by osteoblasts and osteoclasts. For decalcified paraffin-embedded specimens, decalcifying agents alter the staining intensity, and excess decalcification interferes with bone staining. Robust bone staining methods independent of the decalcification conditions and animal species are lacking. In this study, we have developed a novel polychrome staining method, named JFRL staining, which stains the components of osteochondral tissue in different colors. With this staining we could visualize the hyaline cartilage as blue by alcian blue, osteoid as red by picrosirius red, and mineralized bone as green by picro-light green SF or picro-naphthol green B and easily distinguished osteoblasts, osteocytes, and osteoclasts. In mineralized bone, this staining revealed the obvious lamellar structures and woven bone. Notably, this staining was independent of the decalcification conditions and experimental animal species examined. To verify the usefulness of JFRL staining, we observed cotton rat tail which has shorter length and shows a false autotomy. The caudal vertebrae were normally developed via endochondral ossification without a fracture plane. At 6 months of age, the number of chondrocytes declined and the hypertrophic zone was absent at the epiphyseal plate, which might reflect the shorter tail. In conclusion, JFRL staining is the first method to simultaneously distinguish osteochondral matrix and bone cells in one section regardless of decalcifying conditions. This robust staining will provide new information for a wide number of biomedical fields, including bone development, physiology, and pathology.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	This is a post-peer-review, pre-copyedit version of an article published in Cell and tissue research. The final authenticated version is available online at: http://doi.org/10.1007/s00441-021-03516-6
PISSN	0302-766X
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1007/s00441-021-03516-6