open access

https://hdl.handle.net/2115/86672

Methyloradius palustris gen. nov., sp. nov., a methanol-oxidizing bacterium isolated from snow

英語

novel genus

2021-09-03

Springer

Archives of Microbiology

203

5715-5721

A novel methylotrophic bacterium, strain Zm11(T), was isolated from reddish brown snow collected in a moor in Japan. Cells of the isolate were Gram-stain-negative, motile, and rod-shaped (0.6-0.7 x 1.2-2.7 mu m). Growth was observed at 5-32 degrees C with an optimum growth temperature of 25-28 degrees C. The pH range for growth was 5.4-7.8 with an optimum pH of 6.8. The strain utilized only methanol as carbon and energy sources for aerobic growth. The major cellular fatty acids (> 40% of total) were summed feature 3 (C-16:1 omega 7c and/or C-16:1 omega 6c) and C-16: 0. The predominant quinone was Q-8, and major polar lipids were phosphatidylethanolamine and phosphatidylglycerol. The complete genome of strain Zm11(T) is composed of a circular chromosome (2,800,413 bp), with G + C content of 46.4 mol%. Phylogenetic analyses were conducted based on the 16S rRNA gene sequence and conserved proteins encoded in the genome. The results of analyses indicate that strain Zm11(T) is a member of the family Methylophilaceae but does not belong to any existing genus. On the basis of its genomic and phenotypic properties, strain Zm11(T) (= DSM111909(T) = NBRC114766(T)) is proposed as the type strain of a new species in a new genus, Methyloradius palustris gen. nov., sp. nov.

学術雑誌論文

Accepted Manuscript

This is a post-peer-review, pre-copyedit version of an article published in Archives of Microbiology. The final authenticated version is available online at: http://dx.doi.org/10.1007/s00203-021-02559-1

0302-8933