Usefulness of a 3D-printing air sampler for capturing live airborne bacteria and exploring the environmental factors that can influence bacterial dynamics

Mori, Saaya; Ishiguro, Sakura; Miyazaki, Satoru; Okubo, Torahiko; Omori, Ryosuke; Kai, Ayako; Sugiyama, Kyohei; Kawashiro, Airi; Sumi, Masato; Thapa, Jeewan; Nakamura, Shinji; Katoh, Chietsugu; Yamaguchi, Hiroyuki

Usefulness of a 3D-printing air sampler for capturing live airborne bacteria and exploring the environmental factors that can influence bacterial dynamics

Mori, Saaya; Ishiguro, Sakura; Miyazaki, Satoru et al.

Research in Microbiology, 2021, 172(6), 103864

Permalink : https://hdl.handle.net/2115/86696

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アクセス権	open access
URI	https://hdl.handle.net/2115/86696
タイトル	Usefulness of a 3D-printing air sampler for capturing live airborne bacteria and exploring the environmental factors that can influence bacterial dynamics
著者	Mori, Saaya ; Ishiguro, Sakura ; Miyazaki, Satoru ; Okubo, Torahiko ; Omori, Ryosuke ; Kai, Ayako ; Sugiyama, Kyohei ; Kawashiro, Airi ; Sumi, Masato ; Thapa, Jeewan ; Nakamura, Shinji ; Katoh, Chietsugu ; Yamaguchi, Hiroyuki
著者名 Mori, Saaya
著者名 Ishiguro, Sakura
著者名 Miyazaki, Satoru
e-Rad_Researcher 90762196 著者名 Okubo, Torahiko
e-Rad_Researcher 10746952 著者名 Omori, Ryosuke
著者名 Kai, Ayako
著者名 Sugiyama, Kyohei
著者名 Kawashiro, Airi
著者名 Sumi, Masato
e-Rad_Researcher 40837449 著者名 Thapa, Jeewan
e-Rad_Researcher 40207882 著者名 Nakamura, Shinji
e-Rad_Researcher 10292012 著者名 Katoh, Chietsugu
e-Rad_Researcher 40221650 著者名 Yamaguchi, Hiroyuki
言語	英語
キーワード	3D printing
	Air sampler
	Air bacteria
	Protozoa
	Environmental factors
発行日	2021-09-01
出版者	Elsevier
収録物名	Research in Microbiology
巻(号)	172(6)
ページ	103864
抄録	We created a handmade 3D-printed air sampler to effectively collect live airborne bacteria, and determined which environmental factors influenced the bacteria. Bacterial colony forming units (CFUs) in the air samples (n = 37) were monitored by recording the environmental changes occurring over time, then determining the presence/absence of correlations among such changes. The bacterial CFUs changed sharply and were significantly correlated with the DNA concentrations, indicating that the captured bacteria made up most of the airborne bacteria. Spearman's rank correlation analysis revealed significant correlations between the bacterial CFU values and some environmental factors (humidity, wind speed, insolation, and 24-h rainfall). Similarly the significant associations of CFU with humidity and wind speed were also found by multiple regression analysis with box-cox transformation. Among our panel of airborne bacteria (952 strains), 70 strains were identified as soil-derived Bacillus via the production of Escherichia coli- and Staphylococcus aureus-growth inhibiting antibiotics and by 16S rDNA typing. Soil-derived protozoa were also isolated from the air samples. We conclude that the airborne bacteria mainly derived from soil can alter in number according to environmental changes. Our sampler, which was created by easy-to-customize 3D printing, is a useful device for understanding the dynamics of live airborne bacteria. (C) 2021 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
権利情報	© 2021. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/
PISSN	0923-2508
関連情報	PMID 34273486
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.resmic.2021.103864