Intracerebral Transplantation of Mesenchymal Stromal Cell Compounded with Recombinant Peptide Scaffold against Chronic Intracerebral Hemorrhage Model

Takamiya, Soichiro; Kawabori, Masahito; Kitahashi, Tsukasa et al.

Stem cells international, 2022, 2022, 8521922

Permalink : https://hdl.handle.net/2115/86780

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https://doi.org/10.1155/2022/8521922     4

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アクセス権
metadata only access
URI
https://hdl.handle.net/2115/86780
タイトル
Intracerebral Transplantation of Mesenchymal Stromal Cell Compounded with Recombinant Peptide Scaffold against Chronic Intracerebral Hemorrhage Model
著者
Takamiya, Soichiro ; Kawabori, Masahito ; Kitahashi, Tsukasa ; Nakamura, Kentaro ; Mizuno, Yuki ; Yasui, Hironobu ; Kuge, Yuji ; Tanimori, Aki ; Takamatsu, Yasuyuki ; Yuyama, Kohei ; Shichinohe, Hideo ; Fujimura, Miki
著者名
Takamiya, Soichiro
e-Rad_Researcher
50399870
著者名
Kawabori, Masahito
著者名
Kitahashi, Tsukasa
著者名
Nakamura, Kentaro
著者名
Mizuno, Yuki
著者名
Yasui, Hironobu
著者名
Kuge, Yuji
著者名
Tanimori, Aki
著者名
Takamatsu, Yasuyuki
著者名
Yuyama, Kohei
著者名
Shichinohe, Hideo
e-Rad_Researcher
00361098
著者名
Fujimura, Miki
言語
英語
発行日
2022-07-31
出版者
Hindawi Publishing Corporation
収録物名
Stem cells international
巻(号)
2022
ページ
8521922
抄録
Background. Due to the lack of effective therapies, stem cell transplantation is an anticipated treatment for chronic intracerebral hemorrhage (ICH), and higher cell survival and engraftment are considered to be the key for recovery. Mesenchymal stromal cells (MSCs) compounded with recombinant human collagen type I scaffolds (CellSaics) have a higher potential for cell survival and engraftment compared with solo-MSCs, and we investigated the validity of intracerebral transplantation of CellSaic in a chronic ICH model. Methods. Rat CellSaics (rCellSaics) were produced by rat bone marrow-derived MSC (rBMSCs). The secretion potential of neurotrophic factors and the cell proliferation rate were compared under oxygen-glucose deprivation (OGD) conditions. rCellSaics, rBMSCs, or saline were transplanted into the hollow cavity of a rat chronic ICH model. Functional and histological analyses were evaluated, and single-photon emission computed tomography for benzodiazepine receptors was performed to monitor sequential changes in neuronal integrity. Furthermore, human CellSaics (hCellSaics) were transplanted into a chronic ICH model in immunodeficient rats. Antibodies neutralizing brain-derived neurotrophic factor (BDNF) were used to elucidate its mode of action. Results. rCellSaics demonstrated a higher secretion potential of trophic factors and showed better cell proliferation in the OGD condition. Animals receiving rCellSaics displayed better neurological recovery, higher intracerebral BDNF, and better cell engraftment; they also showed a tendency for less brain atrophy and higher benzodiazepine receptor preservation. hCellSaics also promoted significant functional recovery, which was reversed by BDNF neutralization. Conclusion. Intracerebral transplantation of CellSaics enabled neurological recovery in a chronic ICH model and may be a good option for clinical application.
資源タイプ
学術雑誌論文
出版タイプ
Version of Record
PISSN
1687-966X
関連情報 (isIdenticalTo)
出版社版DOI
https://doi.org/10.1155/2022/8521922