Analysis of the Pancreatic Cancer Microbiome Using Endoscopic Ultrasound-Guided Fine-Needle Aspiration-Derived Samples

Nakano, Shintaro; Kawamoto, Yasuyuki; Komatsu, Yoshito et al.

Pancreas, 2022, 51(4), 351-357

Permalink : https://hdl.handle.net/2115/86784

このアイテムのアクセス数:357件（2026-08-17 20:15 集計）

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https://doi.org/10.1097/MPA.0000000000002028     37

論文情報

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アクセス権
open access
URI
https://hdl.handle.net/2115/86784
タイトル
Analysis of the Pancreatic Cancer Microbiome Using Endoscopic Ultrasound-Guided Fine-Needle Aspiration-Derived Samples
著者
Nakano, Shintaro ; Kawamoto, Yasuyuki ; Komatsu, Yoshito ; Saito, Rika ; Ito, Ken ; Yamamura, Takahiro ; Harada, Kazuaki ; Yuki, Satoshi ; Kawakubo, Kazumichi ; Sugiura, Ryo ; Kato, Shin ; Hirata, Koji ; Hirata, Hajime ; Nakajima, Masahito ; Furukawa, Ryutaro ; Takishin, Yunosuke ; Nagai, Kousuke ; Yokota, Isao ; Ota, Keisuke H. ; Nakaoka, Shinji ; Kuwatani, Masaki ; Sakamoto, Naoya
著者名
Nakano, Shintaro
e-Rad_Researcher
60755601
著者名
Kawamoto, Yasuyuki
e-Rad_Researcher
60333598
著者名
Komatsu, Yoshito
著者名
Saito, Rika
著者名
Ito, Ken
著者名
Yamamura, Takahiro
著者名
Harada, Kazuaki
著者名
Yuki, Satoshi
e-Rad_Researcher
80633578
著者名
Kawakubo, Kazumichi
著者名
Sugiura, Ryo
著者名
Kato, Shin
著者名
Hirata, Koji
著者名
Hirata, Hajime
著者名
Nakajima, Masahito
著者名
Furukawa, Ryutaro
著者名
Takishin, Yunosuke
著者名
Nagai, Kousuke
e-Rad_Researcher
20761414
著者名
Yokota, Isao
著者名
Ota, Keisuke H.
著者名
Nakaoka, Shinji
e-Rad_Researcher
50431375
著者名
Kuwatani, Masaki
e-Rad_Researcher
10334418
著者名
Sakamoto, Naoya
言語
英語
キーワード
endoscopic ultrasound-guided fine-needle aspiration
bacteria
microbiome
pancreas
pancreatic cancer
発行日
2022-04
出版者
Lippincott Williams & Wilkins (LWW)
収録物名
Pancreas
巻(号)
51(4)
ページ
351-357
抄録
Objectives Most previous studies have analyzed bacteria in tumors using resected pancreatic cancer (PC) tissues, because it is difficult to obtain tissue samples from unresectable advanced PC. We aimed to determine whether minimal tissue obtained by endoscopic ultrasound-guided fine-needle aspiration is useful for microbiome analysis. Methods Thirty PC and matched duodenal and stomach tissues (N = 90) were prospectively collected from 30 patients who underwent endoscopic ultrasound-guided fine-needle aspiration. Bacterial DNA was extracted, and 16S rRNA sequencing was performed. The primary outcome was the success rate of bacterial detection in tumors. Bacterial diversity and structure were investigated. Results The bacterial detection rates were 80%, 100%, and 97% in PC, gastric, and duodenal samples, respectively. Pancreatic cancer tissues showed a lower alpha-diversity and a significantly different microbial structure than stomach and duodenal tissues. Proteobacteria were more abundant, whereas Firmicutes, Bacteroidetes, and Fusobacteria were less abundant in PC tissues than in stomach and duodenal tissues. Acinetobacter was more abundant in PC tissues than in stomach and duodenal tissues, and Delftia was more frequently detected in resectable PC. Conclusions Endoscopic ultrasound-guided fine-needle aspiration samples were valuable for PC microbiome analysis, revealing that the bacterial composition of PC is different from that of the stomach and duodenum.
資源タイプ
学術雑誌論文
出版タイプ
Version of Record
PISSN
0885-3177
関連情報 (isIdenticalTo)
出版社版DOI
https://doi.org/10.1097/MPA.0000000000002028