A method for the generation of pseudovirus particles bearing SARS coronavirus spike protein in high yields

Fujioka, Yoichiro; Kashiwagi, Sayaka; Yoshida, Aiko; Satoh, Aya O.; Fujioka, Mari; Amano, Maho; Yamauchi, Yohei; Ohba, Yusuke

A method for the generation of pseudovirus particles bearing SARS coronavirus spike protein in high yields

Fujioka, Yoichiro; Kashiwagi, Sayaka; Yoshida, Aiko et al.

Cell structure and function, 2022, 47(1), 43-53

Permalink : https://hdl.handle.net/2115/86796

このアイテムのアクセス数:488件（2026-08-24 19:46 集計）

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論文情報

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アクセス権	open access
URI	https://hdl.handle.net/2115/86796
タイトル	A method for the generation of pseudovirus particles bearing SARS coronavirus spike protein in high yields
著者	Fujioka, Yoichiro ; Kashiwagi, Sayaka ; Yoshida, Aiko ; Satoh, Aya O. ; Fujioka, Mari ; Amano, Maho ; Yamauchi, Yohei ; Ohba, Yusuke
e-Rad_Researcher 70597492 著者名 Fujioka, Yoichiro
著者名 Kashiwagi, Sayaka
著者名 Yoshida, Aiko
著者名 Satoh, Aya O.
著者名 Fujioka, Mari
著者名 Amano, Maho
著者名 Yamauchi, Yohei
e-Rad_Researcher 30333503 著者名 Ohba, Yusuke
言語	英語
キーワード	severe acute respiratory syndrome coronavirus (SARS-CoV)
	SARS-CoV-2
	pseudovirus
	vesicular stomatitis virus (VSV)
	spike protein
発行日	2022-04-28
出版者	一般社団法人日本細胞生物学会(Japan Society for Cell Biology)
収録物名	Cell structure and function
巻(号)	47(1)
ページ	43-53
抄録	The ongoing severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has threatened human health and the global economy. Development of additional vaccines and therapeutics is urgently required, but such development with live virus must be conducted with biosafety level 3 confinement. Pseudotyped viruses have been widely adopted for studies of virus entry and pharmaceutical development to overcome this restriction. Here we describe a modified protocol to generate vesicular stomatitis virus (VSV) pseudotyped with SARS-CoV or SARSCoV-2 spike protein in high yield. We found that a large proportion of pseudovirions produced with the conventional transient expression system lacked coronavirus spike protein at their surface as a result of inhibition of parental VSV infection by overexpression of this protein. Establishment of stable cell lines with an optimal expression level of coronavirus spike protein allowed the efficient production of progeny pseudoviruses decorated with spike protein. This improved VSV pseudovirus production method should facilitate studies of coronavirus entry and development of antiviral agents.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
PISSN	0386-7196
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1247/csf.21047