In vitro characterization of adipocyte plasma membrane-associated protein from poultry red mites, Dermanyssus gallinae, as a vaccine antigen for chickens

Fujisawa, Sotaro; Murata, Shiro; Takehara, Masaki; Aoyama, Julia; Morita, Ayu; Isezaki, Masayoshi; Win, Shwe Yee; Ariizumi, Takuma; Sato, Takumi; Oishi, Eiji; Taneno, Akira; Maekawa, Naoya; Okagawa, Tomohiro; Ichii, Osamu; Konnai, Satoru; Ohashi, Kazuhiko

In vitro characterization of adipocyte plasma membrane-associated protein from poultry red mites, Dermanyssus gallinae, as a vaccine antigen for chickens

Fujisawa, Sotaro; Murata, Shiro; Takehara, Masaki et al.

Vaccine, 2021, 39(41), 6057-6066

Permalink : https://hdl.handle.net/2115/86867

このアイテムのアクセス数:515件（2026-08-11 19:54 集計）

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アクセス権	open access
URI	https://hdl.handle.net/2115/86867
タイトル	In vitro characterization of adipocyte plasma membrane-associated protein from poultry red mites, Dermanyssus gallinae, as a vaccine antigen for chickens
著者	Fujisawa, Sotaro ; Murata, Shiro ; Takehara, Masaki ; Aoyama, Julia ; Morita, Ayu ; Isezaki, Masayoshi ; Win, Shwe Yee ; Ariizumi, Takuma ; Sato, Takumi ; Oishi, Eiji ; Taneno, Akira ; Maekawa, Naoya ; Okagawa, Tomohiro ; Ichii, Osamu ; Konnai, Satoru ; Ohashi, Kazuhiko
著者名 Fujisawa, Sotaro
e-Rad_Researcher 10579163 著者名 Murata, Shiro
著者名 Takehara, Masaki
著者名 Aoyama, Julia
著者名 Morita, Ayu
著者名 Isezaki, Masayoshi
著者名 Win, Shwe Yee
著者名 Ariizumi, Takuma
著者名 Sato, Takumi
著者名 Oishi, Eiji
著者名 Taneno, Akira
e-Rad_Researcher 70829035 著者名 Maekawa, Naoya
e-Rad_Researcher 80829036 著者名 Okagawa, Tomohiro
e-Rad_Researcher 60547769 著者名 Ichii, Osamu
e-Rad_Researcher 40396304 著者名 Konnai, Satoru
e-Rad_Researcher 90250498 著者名 Ohashi, Kazuhiko
言語	英語
キーワード	Poultry red mite
	Adipocyte plasma membrane-associated protein
	Vaccine
	Dg-APMAP
	Dermanyssus gallinae
発行日	2021-10-01
出版者	Elsevier
収録物名	Vaccine
巻(号)	39(41)
ページ	6057-6066
抄録	The poultry red mite (Dermanyssus gallinae; PRM) is a blood-sucking ectoparasite of chickens that is a threat to poultry farming worldwide and significantly reduces productivity in the egg-laying industry. Chemical acaricides that are widely used in poultry farms for the prevention of PRMs are frequently ineffective due to the emergence of acaricide-resistant PRMs. Therefore, alternative control methods are needed, and vaccination is a promising strategy for controlling PRMs. A novel adipocyte-plasma membrane-associated protein-like molecule (Dg-APMAP) is highly expressed in blood-fed PRMs according to a previous RNA sequencing analysis. Here, we attempted to identify the full sequence of DgAPMAP, study its expression in different life stages of PRMs, and evaluate its potential as a vaccine antigen. Dg-APMAP mRNA was expressed in the midgut and ovaries, and in all life stages regardless of feeding states. Importantly, in vitro feeding of PRMs with plasma derived from chickens immunized with the recombinant protein of the extracellular region of Dg-APMAP significantly reduced their survival rate in nymphs and adults, which require blood meals. Our data suggest that the host immune responses induced by vaccination with Dg-APMAP could be an effective strategy to reduce the suffering caused by PRMs in the poultry industry. (c) 2021 Elsevier Ltd. All rights reserved.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
権利情報	© 2021. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
PISSN	0264-410X
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.vaccine.2021.08.104