Obese status is associated with accelerated DNA methylation change in peripheral blood of senior dogs

Yamazaki, Jumpei; Meagawa, Shinji; Jelinek, Jaroslav; Yokoyama, Shoko; Nagata, Noriyuki; Yuki, Masashi; Takiguchi, Mitsuyoshi

Obese status is associated with accelerated DNA methylation change in peripheral blood of senior dogs

Yamazaki, Jumpei; Meagawa, Shinji; Jelinek, Jaroslav et al.

Research in veterinary science, 2021, 139, 193-199

Permalink : https://hdl.handle.net/2115/86894

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アクセス権	open access
URI	https://hdl.handle.net/2115/86894
タイトル	Obese status is associated with accelerated DNA methylation change in peripheral blood of senior dogs
著者	Yamazaki, Jumpei ; Meagawa, Shinji ; Jelinek, Jaroslav ; Yokoyama, Shoko ; Nagata, Noriyuki ; Yuki, Masashi ; Takiguchi, Mitsuyoshi
e-Rad_Researcher 20732902 著者名 Yamazaki, Jumpei
著者名 Meagawa, Shinji
著者名 Jelinek, Jaroslav
著者名 Yokoyama, Shoko
著者名 Nagata, Noriyuki
著者名 Yuki, Masashi
e-Rad_Researcher 70261336 著者名 Takiguchi, Mitsuyoshi
言語	英語
キーワード	DNA methylation
	Epigenetics
	Genome-wide profiling
	Obesity
	Dog
発行日	2021-07-24
出版者	Elsevier
収録物名	Research in veterinary science
巻(号)	139
ページ	193-199
抄録	Obesity and its associated comorbidities constitute a major and growing health problem worldwide not only involved with people but also dogs and cats. Although few genetic mutations have been associated with obesity in dogs, molecular mechanism remains to be clearly understood. Given the fact that DNA methylation leads to gene expression variability and has plasticity affected by metabolic phenotypes such as obesity in human, the objective of this study is to identify obesity-associated differentially methylated cytosine-phosphate-guanine (CpG) dinucleotide sites in dogs. With genome-wide DNA methylation analysis using next-generation sequencing for blood samples from fourteen Miniature dachshunds with body condition score (BCS) 4-5 and BCS >6, over 100,000 sites could be analysed to identify genomic locations of differentially methylated CpG sites. As a result, 191 differentially methylated CpG sites (89 CpG sites were hypermethylated in BCS >6 and 102 were hypermethylated in BCS 4-5) were identified. These sites included promoter regions of Kisspeptin receptor (KISS1R) and Calcyphosine 2 (CAPS2) genes which were subsequently validated by bisulfite-pyrosequencing for another set of 157 dog blood samples. KISS1R methylation levels were found to be higher in BCS >6 group than BCS 4-5 in senior (>84 months) dogs. Especially male dogs but not female dogs as well as uncastrated male dogs but not castrated male dogs showed this trend. DNA methylation of KISS1R gene will be useful for understanding of comprehensive epigenetic change in obese dogs.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
権利情報	© 2021. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/
PISSN	0034-5288
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.rvsc.2021.07.024