Small-molecule inhibitor cocktail promotes the proliferation of pre-existing liver progenitor cells

Fu, Qingjie; Ohnishi, Shunsuke; Suda, Goki et al.

Stem Cell Reports, 2022, 17(7), 1589-1603

Permalink : https://hdl.handle.net/2115/86991

このアイテムのアクセス数:502件（2026-03-13 20:43 集計）

閲覧可能ファイル 

ファイル フォーマット サイズ 閲覧回数 説明
https://doi.org/10.1016/j.stemcr.2022.05.023     2

論文情報

ファイル出力 EndNote Basic出力 Mendeley出力

アクセス権
metadata only access
URI
https://hdl.handle.net/2115/86991
タイトル
Small-molecule inhibitor cocktail promotes the proliferation of pre-existing liver progenitor cells
著者
Fu, Qingjie ; Ohnishi, Shunsuke ; Suda, Goki ; Sakamoto, Naoya
著者名
Fu, Qingjie
e-Rad_Researcher
10443475
著者名
Ohnishi, Shunsuke
e-Rad_Researcher
20447460
著者名
Suda, Goki
e-Rad_Researcher
10334418
著者名
Sakamoto, Naoya
言語
英語
発行日
2022-07-12
出版者
Cell Press
収録物名
Stem Cell Reports
巻(号)
17(7)
ページ
1589-1603
抄録
A recent study showed that a cocktail of three small molecules, Y-27632, A83-01, and CHIR99021 (YAC), converts mature hepatocytes (MHs) into proliferative bipotent cells that can be induced into MHs and cholangiocytes in rats. However, when we reproduced these experiments, it was found that bipotent cells may be derived from resident liver progenitor cells (LPCs), whose proliferative activity was promoted by YAC. A simple and efficient sorting scheme was also developed in this study to harvest high-purity and high-yield LPCs. The inducible bipotency of purified LPCs was verified; in addition, they were found to spontaneously differentiate into hepatocytes and cholangiocytes due to changes in proliferative status even without induction. Moreover, during the differentiation process, some hepatocytes spontaneously reconverted to LPCs under certain conditions, such as the release of contact inhibition. These findings may improve our understanding of LPCs and provide a cell source for regenerative medicine.
資源タイプ
学術雑誌論文
出版タイプ
Version of Record
PISSN
2213-6711
関連情報 (isIdenticalTo)
出版社版DOI
https://doi.org/10.1016/j.stemcr.2022.05.023