Evaluation of Plant Ceramide Species-Induced Exosome Release from Neuronal Cells and Exosome Loading Using Deuterium Chemistry

Murai, Yuta; Honda, Takumi; Yuyama, Kohei et al.

International Journal of Molecular Sciences, 2022, 23(18), 10751

Permalink : https://hdl.handle.net/2115/87021

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https://doi.org/10.3390/ijms231810751     98

論文情報

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アクセス権
open access
URI
https://hdl.handle.net/2115/87021
タイトル
Evaluation of Plant Ceramide Species-Induced Exosome Release from Neuronal Cells and Exosome Loading Using Deuterium Chemistry
著者
Murai, Yuta ; Honda, Takumi ; Yuyama, Kohei ; Mikami, Daisuke ; Eguchi, Koichi ; Ukawa, Yuichi ; Usuki, Seigo ; Igarashi, Yasuyuki ; Monde, Kenji
e-Rad_Researcher
20707038
著者名
Murai, Yuta
著者名
Honda, Takumi
著者名
Yuyama, Kohei
著者名
Mikami, Daisuke
著者名
Eguchi, Koichi
著者名
Ukawa, Yuichi
著者名
Usuki, Seigo
著者名
Igarashi, Yasuyuki
著者名
Monde, Kenji
言語
英語
キーワード
plant ceramide
exosome
amyloid-beta
Alzheimer's disease
lipidomics
deuterium
発行日
2022-09-15
出版者
MDPI
収録物名
International Journal of Molecular Sciences
巻(号)
23(18)
ページ
10751
抄録
The extracellular accumulation of aggregated amyloid-beta (A beta) in the brain leads to the early pathology of Alzheimer's disease (AD). The administration of exogenous plant-type ceramides into AD model mice can promote the release of neuronal exosomes, a subtype of extracellular vesicles, that can mediate A beta clearance. In vitro studies showed that the length of fatty acids in mammalian-type ceramides is crucial for promoting neuronal exosome release. Therefore, investigating the structures of plant ceramides is important for evaluating the potential in releasing exosomes to remove A beta. In this study, we assessed plant ceramide species with D-erythro-(4E,8Z)-sphingadienine and D-erythro-(8Z)-phytosphingenine as sphingoid bases that differ from mammalian-type species. Some plant ceramides were more effective than mammalian ceramides at stimulating exosome release. In addition, using deuterium chemistry-based lipidomics, most exogenous plant ceramides were confirmed to be derived from exosomes. These results suggest that the ceramide-dependent upregulation of exosome release may promote the release of exogenous ceramides from cells, and plant ceramides with long-chain fatty acids can effectively release neuronal exosomes and prevent AD pathology.
資源タイプ
学術雑誌論文
出版タイプ
Version of Record
関連情報 (isIdenticalTo)
出版社版DOI
https://doi.org/10.3390/ijms231810751